|
|
 Previous Article | Table of Contents | Next Article 
Absence of bcl-2 expression by activated CD45RO+ T lymphocytes in acute
infectious mononucleosis supporting their susceptibility to programmed cell
death
Y Tamaru, T Miyawaki, K Iwai, T Tsuji, R Nibu, A Yachie, S Koizumi and N Taniguchi
Department of Pediatrics, School of Medicine, Kanazawa University, Japan.
bcl-2 proto-oncogene encodes an inner mitochondrial membrane protein that
blocks programmed cell death (apoptosis). There is now increasing evidence
that regulation of bcl-2 expression is a determinant of life or death in
normal lymphocytes. We have recently described that activated (CD45RO+)
CD4+ and CD8+ T cells in acute infectious mononucleosis (IM) undergo
apoptotic cell death on culturing, indicating an activation-driven cell
death of mature T cells. In this work, we examine bcl-2 expression by
activated T cells in acute IM using a flow-cytometric analysis with an
anti-bcl-2 monoclonal antibody (MoAb). It was consistently observed that
most T cells from acute IM patients displayed only much less bcl-2, while
normal T cells expressed bcl-2 relatively strongly. Multicolor analysis
showed that bcl-2- lacking T cells in acute IM were restricted to the
CD45RO+ (activated) populations of CD4+, as well as CD8+ T cells. In
contrast, the relatively intense levels of bcl-2 were expressed in both
CD45RO+ and CD45RO- T-cell populations from normal subjects. This marked
difference in bcl-2 expression of CD45RO+ T cells between acute IM and
normal controls was also confirmed by Western blot analysis. Activated
(CD45RO+) T cells with low bcl-2 expression, but not bcl-2-expressing
CD45RO- T cells, in acute IM patients were found to die easily when
cultured without added growth factors. However, in normal individuals, both
CD45RO+ and CD45RO- T cells were relatively stable on culturing. These
findings suggest that lack of bcl-2 expression by activated (CD45RO+) T
cells in acute IM might be associated with their susceptibility to
programmed cell death.
Volume 82,
Issue 2,
pp. 521-527,
07/15/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
Y. Kasahara, A. Yachie, K. Takei, C. Kanegane, K. Okada, K. Ohta, H. Seki, N. Igarashi, K. Maruhashi, K. Katayama, et al.
Differential cellular targets of Epstein-Barr virus (EBV) infection between acute EBV-associated hemophagocytic lymphohistiocytosis and chronic active EBV infection
Blood,
September 15, 2001;
98(6):
1882 - 1888.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J Itoh, C de la Motte, S A Strong, A D Levine, and C Fiocchi
Decreased Bax expression by mucosal T cells favours resistance to apoptosis in Crohn's disease
Gut,
July 1, 2001;
49(1):
35 - 41.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Tu, P.-T. Cheung, and Y.-L. Lau
Insulin-Like Growth Factor 1 Promotes Cord Blood T Cell Maturation and Inhibits Its Spontaneous and Phytohemagglutinin-Induced Apoptosis Through Different Mechanisms
J. Immunol.,
August 1, 2000;
165(3):
1331 - 1336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Tanner and C. Alfieri
Epstein-Barr Virus Induces Fas (CD95) in T Cells and Fas Ligand in B Cells Leading to T-Cell Apoptosis
Blood,
November 15, 1999;
94(10):
3439 - 3447.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. C. Hebib, O. Deas, M. Rouleau, A. Durrbach, B. Charpentier, F. Beaujean, J.-P. Vernant, and A. Senik
Peripheral Blood T Cells Generated After Allogeneic Bone Marrow Transplantation: Lower Levels of Bcl-2 Protein and Enhanced Sensitivity to Spontaneous and CD95-Mediated Apoptosis In Vitro. Abrogation of the Apoptotic Phenotype Coincides With the Recovery of Normal Naive/Primed T-Cell Profiles
Blood,
September 1, 1999;
94(5):
1803 - 1813.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Mathew, I. Kurane, S. Green, D. W. Vaughn, S. Kalayanarooj, S. Suntayakorn, F. A. Ennis, and A. L. Rothman
Impaired T Cell Proliferation in Acute Dengue Infection
J. Immunol.,
May 1, 1999;
162(9):
5609 - 5615.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Kawai
From Myocarditis to Cardiomyopathy: Mechanisms of Inflammation and Cell Death : Learning From the Past for the Future
Circulation,
March 2, 1999;
99(8):
1091 - 1100.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Vieira, D. Soares, N. J. Borthwick, M. K. Maini, G. Janossy, M. Salmon, and A. N. Akbar
IL-7-Dependent Extrathymic Expansion of CD45RA+ T Cells Enables Preservation of a Naive Repertoire
J. Immunol.,
December 1, 1998;
161(11):
5909 - 5917.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Futatani, T. Miyawaki, S. Tsukada, S. Hashimoto, T. Kunikata, S. Arai, M. Kurimoto, Y. Niida, H. Matsuoka, Y. Sakiyama, et al.
Deficient Expression of Bruton's Tyrosine Kinase in Monocytes From X-Linked Agammaglobulinemia as Evaluated by a Flow Cytometric Analysis and Its Clinical Application to Carrier Detection
Blood,
January 15, 1998;
91(2):
595 - 602.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Matsuzaki, K.-i. Nakayama, K. Nakayama, T. Tomita, M. Isoda, D. Y. Loh, and H. Nakauchi
Role of bcl-2 in the Development of Lymphoid Cells From the Hematopoietic Stem Cell
Blood,
February 1, 1997;
89(3):
853 - 862.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Basso, G. Thiene, D. Corrado, A. Angelini, A. Nava, and M. Valente
Arrhythmogenic Right Ventricular Cardiomyopathy: Dysplasia, Dystrophy, or Myocarditis?
Circulation,
September 1, 1996;
94(5):
983 - 991.
[Abstract]
[Full Text]
|
|
|
|
|
