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Identification of yeast artificial chromosomes containing the inversion 16
p-arm breakpoint associated with acute myelomonocytic leukemia
P Liu, DF Claxton, P Marlton, A Hajra, J Siciliano, M Freedman, SC Chandrasekharappa, K Yanagisawa, RL Stallings and FS Collins
Department of Hematology, University of Texas M.D. Anderson Cancer Center,
Houston 77030.
We report the cloning of the chromosome 16 p-arm breakpoint involved in
inversion 16(p13;q22) associated with subtype of acute myelomonocytic
leukemia (AMML) M4Eo. Inter-Alu polymerase chain reaction (PCR) products
from a series of interspecific somatic cell hybrids that contain only small
portions of the human chromosome 16 p-arm were generated for use as
fluorescent in-situ hybridization (FISH) probes. When applied to patient
cells, rapid and unambiguous identification of the inversion resulted.
Using FISH analysis, cosmid clones associated with the hybrids were
identified that bracketed the p-arm breakpoint. A repeat-free fragment of
one of these cosmids (35B11) when used as probe on Southern blots from
pulsed-field gels identified rearranged macrorestriction fragments in
patient DNA. Yeast artificial chromosomes (YACs) were isolated using
sequences derived from cosmids flanking 35B11 in a cosmid contig. Of 4 YACs
so identified, 3 were shown by FISH to cross the inversion-16 p-arm
breakpoint. Therefore, the breakpoint has been molecularly cloned, and
identified as being within these 3 YACs. These clones will facilitate the
unraveling of the genetic events associated with inversion-16 and are
available tools with immediate clinical application.
Volume 82,
Issue 3,
pp. 716-721,
08/01/1993
Copyright © 1993 by The American Society of Hematology
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