Ontogeny of hematopoietic stem cell development: reciprocal expression of
CD33 and a novel molecule by maturing myeloid and erythroid progenitors
C Brashem-Stein, DA Flowers, FO Smith, SJ Staats, RG Andrews and ID Bernstein
Programs in Pediatric Oncology, Fred Hutchinson Cancer Research Center,
Seattle, WA 98104.
We have identified a molecule expressed by human marrow
granulocyte/monocyte colony-forming cells (CFU-GM), erythroid colony-
forming cells (CFU-E), and erythroid burst-forming units (BFU-E), but not
their precursors detectable in long-term bone marrow culture. This antigen,
detected by flow microfluorimetry using monoclonal antibody 7B9, is
coexpressed with CD33 on many CD34+ CFCs, although only the 7B9 antigen was
detected on a portion of BFU-E and CFU-E, whereas only CD33 was found on a
portion of CFU-GM. Antibody 7B9 appears to be useful for isolating subsets
of progenitors based on their common or selective expression of 7B9 antigen
and CD33.
Volume 82,
Issue 3,
pp. 792-799,
08/01/1993
Copyright © 1993 by The American Society of Hematology
