|
|
 Previous Article | Table of Contents | Next Article 
Fibrinogen potentiates the effect of interleukin-3 on early human
hematopoietic progenitors
YQ Zhou, JP Levesque, A Hatzfeld, AA Cardoso, ML Li, P Sansilvestri and J Hatzfeld
Laboratoire de Biologie Cellulaire et Moleculaire des Facteurs de
Croissance, Centre National de la Recherche Scientifique, Villejuif,
France.
The effect of human fibrinogen on the proliferation of purified SBA- CD34+
human bone marrow progenitors was investigated in clonal cultures.
Fibrinogen alone or in combination with erythropoietin had no significant
effect. However, in the presence of recombinant human interleukin-3 (IL-3),
fibrinogen increased significantly in a dose- dependent manner the number
of mixed and burst-forming unit-ethrocyte-- derived colonies, whereas the
number of other colonies did not significantly change. In the presence of
fibrinogen, low concentrations of IL-3 (0.17 U/mL) produced three times
more mixed colonies than without fibrinogen, reaching the number of
colonies obtained with optimal concentrations of IL-3 (1.67 U/mL).
Fibrinogen fragment D had the same effect in the presence of IL-3 as intact
fibrinogen, whereas fibrinogen fragment E and human collagen IV did not.
This effect was not mediated by integrins, because peptides or monoclonal
antibodies that block fibrinogen binding on integrins alpha IIb beta 3,
alpha v beta 3 (RGD-peptides), alpha m beta 2 (OKM-1), and alpha x beta 2
(HC1/1) did not affect the observed mitogenic effect. The mitogenic effect
of fibrinogen and its D fragment was not mediated by induction of IL-6 or
granulocyte--colony-stimulating factor secretion, because it was not
inhibited by blocking antisera against these two growth factors. Our
results indicate that fibrinogen potentiates the effect of IL-3 on
primitive hematopoietic progenitors and suggest that the mitogenic effect
of fibrinogen could be mediated via a specific mitogenic receptor that does
not belong to the integrin family.
Volume 82,
Issue 3,
pp. 800-806,
08/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. D. Pelletier, D. S. Hong, Y. Hu, Y. Liu, and S. Li
Lack of the adhesion molecules P-selectin and intercellular adhesion molecule-1 accelerate the development of BCR/ABL-induced chronic myeloid leukemia-like myeloproliferative disease in mice
Blood,
October 1, 2004;
104(7):
2163 - 2171.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kramer, S. Horner, A. Willer, S. Fruehauf, A. Hochhaus, M. Hallek, and R. Hehlmann
Adhesion to fibronectin stimulates proliferation of wild-type and bcr/abl-transfected murine hematopoietic cells
PNAS,
March 2, 1999;
96(5):
2087 - 2092.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. P. Schofield, M. J. Humphries, E. de Wynter, N. Testa, and J. T. Gallagher
The Effect of alpha 4beta 1-Integrin Binding Sequences of Fibronectin on Growth of Cells From Human Hematopoietic Progenitors
Blood,
May 1, 1998;
91(9):
3230 - 3238.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. E. Gardiner and S. E. D'Souza
A Mitogenic Action for Fibrinogen Mediated through Intercellular Adhesion Molecule-1
J. Biol. Chem.,
June 13, 1997;
272(24):
15474 - 15480.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
