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mb-1: a new marker for B-lineage lymphoblastic leukemia
V Buccheri, B Mihaljevic, E Matutes, MJ Dyer, DY Mason and D Catovsky
Academic Department of Haematology and Cytogenetics, Royal Marsden
Hospital, London, UK.
The expression of the Ig-linked mb-1 polypeptide was analyzed by
immunocytochemistry (alkaline phosphatase anti-alkaline phosphatase
technique) using a specific monoclonal antibody in 165 cases of acute
leukemia, with 88 being lymphoblastic (ALL) and 77 myeloid (AML). The
purpose of the study was to investigate the specificity of this reagent for
B-lineage cases and its reactivity on leukemias that coexpress myeloid and
B-cell antigens (biphenotypic). The majority (89%) of 72 B- cell precursor
ALL patients were positive. Of these, mb-1 was expressed in all 9 patients
with early-B-ALL (CD10-, c mu-), in all 11 patients with pre-B-ALL (c mu+)
and in the single case of B-ALL (smIgM+). Forty- three of 51 patients with
common-ALL (CD10+, c mu+) were also positive. All 16 T-lineage ALL patients
and 72 (93.5%) of the AML patients examined were mb-1 negative. Four of the
5 mb-1-positive AML patients were considered biphenotypic and expressed
other B-cell antigens such as CD10, CD19, and/or cCD22 and all showed
rearrangement of the Ig heavy chain genes. Within the AML cases, mb-1 and
cCD22 were more useful than other B-cell antigens in detecting biphenotypic
cases, and mb-1 showed the highest correlation with the clonal
rearrangement of Ig heavy chain genes. These results indicate that mb-1 is
a sensitive and specific reagent for B-lineage blasts that will aid in the
classification of B-cell precursor ALL and in the identification of
biphenotypic leukemia presenting as AML.
Volume 82,
Issue 3,
pp. 853-857,
08/01/1993
Copyright © 1993 by The American Society of Hematology
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