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Demonstration of Epstein-Barr virus replication in Reed-Sternberg cells of
Hodgkin's disease
P Brousset, H Knecht, B Rubin, E Drouet, S Chittal, F Meggetto, TA Saati, E Bachmann, G Denoyel and A Sergeant
Laboratoire d'Anatomie Pathologique, CHU Purpan, Toulouse, France.
Epstein-Barr virus (EBV) is detectable in approximately 40% of cases of
Hodgkin's disease (HD). The viral genomes remain latent but positive
staining with anti-ZEBRA antibody in a small fraction of Reed-Sternberg
(RS) cells of some cases of HD would suggest possible activation of EBV
replication within these cells. We report the investigation of 40 cases of
EBV-associated HD (including 5 human immunodeficiency virus [HIV]- positive
cases) using anti-ZEBRA antibodies. Positive staining was found in only
three (HIV-negative) cases. One of these three cases showed approximately
1% of ZEBRA-positive tumor cells, whereas the other two cases showed rare
positive cells. In the case with 1% ZEBRA- positive cells, a strong signal
was obtained with anti-EA-R antibody and BHLF1 oligoprobes, which indicated
early gene expression. EBV replication could be shown in this case by
nonisotopic in situ DNA-DNA hybridization, which showed markedly increased
numbers of EBV genomes in a few RS cells. Viral replication was confirmed
using reverse transcriptase and polymerase chain reaction that detected
transcripts from the BLLF1 gene encoding for the membrane antigen
gp350/220. EBV replication in RS cells seems to be an exceptional event but
may provide clues to mechanisms of control of viral latency and assume
clinical implications in the future.
Volume 82,
Issue 3,
pp. 872-876,
08/01/1993
Copyright © 1993 by The American Society of Hematology
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