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In situ hybridization on May-Grunwald Giemsa-stained bone marrow and blood
smears of patients with hematologic disorders allows detection of
cell-lineage-specific cytogenetic abnormalities
K van Lom, A Hagemeijer, EM Smit and B Lowenberg
Department of Hematology, University Hospital, Rotterdam, The Netherlands.
Bone marrow and blood from patients with acute myeloid leukemia and
myelodysplastic syndrome were studied by simultaneous analysis of cell
morphology and karyotype. A combined technique of May-Grunwald Giemsa (MGG)
for cell morphology and fluorescence in situ hybridization (FISH) with
chromosome-specific DNA probes for detection of cytogenetic aberrations
allowed us to investigate cell-lineage-specific chromosomal abnormalities.
We introduced video recordings to examine large numbers of cells. Briefly,
evaluation was first performed on MGG slides, during which cell position
and morphology were recorded on an S-VHS recorder. Subsequently, the same
slides were used for FISH. This resulted in the identification of
MGG-stained cells on the video screen and, at the same time, the
interpretation of FISH signals in the fluorescence microscope. Specimens of
bone marrow or blood samples from four patients with different hematologic
malignancies were studied. One of these patients was studied before and
after cytotoxic treatment. The gain or loss of chromosomes could be
detected easily and morphologically assigned to the blasts in all patients
and to a variable proportion of the myelomonocytic lineage in two patients,
but not to the lymphocytes. Thus, this method provides new possibilities
for investigating the clonality of hematologic malignancies.
Volume 82,
Issue 3,
pp. 884-888,
08/01/1993
Copyright © 1993 by The American Society of Hematology
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