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Selectin-mediated rolling of neutrophils on immobilized platelets
SM Buttrum, R Hatton and GB Nash
Department of Haematology, Medical School, University of Birmingham, UK.
Interaction between neutrophils and platelets at the site of vascular
damage or in ischaemic tissue may promote thrombosis and/or vascular
occlusion. To study this interaction, we have developed a novel technique
that allows visualization of adhesion of flowing neutrophils to
immobilized, activated platelets. The total number of adherent neutrophils
decreased with increasing wall shear stress in the range 0.05 to 0.4 Pa.
Although a proportion of the adherent neutrophils were stationary, most
were rolling with a velocity greater than 0.4 micron/s. The percentage of
rolling cells increased with increasing wall shear stress, but the mean
rolling cell velocity was nearly independent of shear stress. Adhesion of
neutrophils was nearly abolished by treatment of the platelets with
antibody to P-selectin, or by treatment of neutrophils with either
neuraminidase, dextran sulfate, or EDTA. Studies with a series of
antibodies to L-selectin (TQ-1, Dreg- 56, LAM1-3, and LAM1-10) suggested
that this molecule was one neutrophil ligand for rolling adhesion. Thus,
sialylated carbohydrate on neutrophils appears essential for
P-selectin-mediated adhesion, and a proportion of this ligand may be
presented by L-selectin. Treatment of the neutrophils with
N-formyl-methionyl-leucyl-phenylalanine decreased the number of rolling
cells, and increased the rolling velocity, possibly due to shedding of
neutrophil ligand(s) and/or cell shape change. In vivo, immobilized
platelets could play an important role in promoting attachment of
neutrophils to vessel walls, eg, by slowing neutrophils so that
integrin-mediated immobilization could occur.
Volume 82,
Issue 4,
pp. 1165-1174,
08/15/1993
Copyright © 1993 by The American Society of Hematology
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