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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shiota, Y. Articles by Tavassoli, M. Search for Related Content PubMed PubMed Citation Articles by Shiota, Y. Articles by Tavassoli, M. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  A novel 37-Kd adhesive membrane protein from cloned murine bone marrow stromal cells and cloned murine hematopoietic progenitor cells Y Shiota, JG Wilson, K Harjes, ED Zanjani and M Tavassoli Department of Veterans Affairs Medical Center, Jackson, MS 39216. The adhesion of hematopoietic progenitor cells to bone marrow stromal cells is critical to hematopoiesis and involves multiple effector molecules. Stromal cell molecules that participate in this interaction were sought by analyzing the detergent-soluble membrane proteins of GBI/6 stromal cells that could be adsorbed by intact FDCP-1 progenitor cells. A single-chain protein from GBI/6 cells having an apparent molecular weight of 37 Kd was selectively adsorbed by FDCP-1 cells. This protein, designated p37, could be surface-radiolabeled and thus appeared to be exposed on the cell membrane. An apparently identical 37- Kd protein was expressed by three stromal cell lines, by Swiss 3T3 fibroblastic cells, and by FDCP-1 and FDCP-2 progenitor cells. p37 was selectively adsorbed from membrane lysates by a variety of murine hematopoietic cells, including erythrocytes, but not by human erythrocytes. Binding of p37 to cells was calcium-dependent, and was not affected by inhibitors of the hematopoietic homing receptor or the cell-binding or heparin-binding functions of fibronectin. It is proposed that p37 may be a novel adhesive molecule expressed on the surface of a variety of hematopoietic cells that could participate in both homotypic and heterotypic interactions of stromal and progenitor cells. Volume 82, Issue 5, pp. 1436-1444, 09/01/1993 Copyright © 1993 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Barda-Saad, A.-S. Zhang, D. Zipori, and L. A. Rozenszajn Adhesion of Thymocytes to Bone Marrow Stromal Cells: Regulation by bFGF and IFN-{gamma} Stem Cells, May 1, 1997; 15(3): 229 - 236. [Abstract] [Full Text]

	Copyright © 1993 by American Society of Hematology         Online ISSN: 1528-0020