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Further study of internal autocrine regulation of multipotent hematopoietic
cells
N Pech, O Hermine and E Goldwasser
Department of Biochemistry and Molecular Biology, University of Chicago, IL
60637.
We have extended the study of the effects of antisense
oligodeoxynucleotides on hematopoietic colony formation to include the
effects of antisense to granulocyte-macrophage colony-stimulating factor
(GM-CSF), granulocyte colony-stimulating factor (G-CSF), and macrophage
colony-stimulating factor (M-CSF) on bone marrow cultures. GM-CSF antisense
and GM-CSF receptor antisense cause an increase in mixed
erythroid:nonerythroid colonies and a decrease in mixed nonerythroid
colonies, which is an effect opposite to that described previously for
erythropoietin (Epo) and Epo receptor antisense. The effect of GM-CSF
antisense oligomer is not abrogated by the presence of the ligand in the
culture. Antisense oligomers to G-CSF and M-CSF have no effect. When Epo
and GM-CSF antisense oligomers are added simultaneously, the effects seem
to be independent, with the GM-CSF antisense predominating. These data
support the hypothesis of internal autocrine regulation of multipotent
hematopoietic precursor cells, and extend the concept to myeloid as well as
erythroid differentiation.
Volume 82,
Issue 5,
pp. 1502-1506,
09/01/1993
Copyright © 1993 by The American Society of Hematology
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