|
|
 Previous Article | Table of Contents | Next Article 
Persistent expansions of CD4+ CD8+ peripheral blood T cells
P Sala, E Tonutti, C Feruglio, F Florian and A Colombatti
Istituto di Chimica Clinica, Ospedale S. Maria della Misericordia, Udine,
Italy.
CD4+ CD8+ cells are present during T cell differentiation in the thymus.
Less than 2% of normal T cells that coexpress CD4 and CD8 also are released
in the circulation and are present in the peripheral blood. In this study,
nine individuals are described that manifested persistent expansions (11%
to 43%) of circulating CD4+ CD8+ T cells that in three cases had large
granular lymphocyte (LGL) morphology in the absence of either lymphocytosis
or overt lymphoproliferative disorders. Southern blot hybridization of
enriched CD4+ CD8+ cells with T-cell receptor beta (TCR beta) and TCR gamma
probes showed that most cases had the 12-kb Eco RI germinal band deleted or
of decreased intensity. In several individuals new TCR beta-specific bands
of different intensity and distinct from case to case suggested either
monoclonal or oligoclonal and polyclonal expansions. Immunophenotypic
analysis showed that in 7 out of 9 cases the CD4+ CD8+ T cells presented
with CD8 dim expression. Furthermore, all the CD4+ CD8+ cells did not
express many of the known activation antigens (low or absent CD25, CD38,
CD71, HLA-DR), whereas they expressed high levels of CD2, CD29, CD56, and
CD57. In addition, the CD4+ CD8+ cells of 5 out of 9 subjects coexpressed
CD45RA and CD45RO suggesting that these cells might be "frozen" in an
intermediate state between naive and memory T cells. In conclusion, the
present CD4+ CD8+ cases fall within a larger spectrum of disorders ranging
from apparently normal to reactive or proliferative situations and
encompassing cells with LGL morphology or LGL-associated antigens
expression either in the presence or in the absence of absolute
lymphocytosis that deserve careful follow-up investigations.
Volume 82,
Issue 5,
pp. 1546-1552,
09/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Nascimbeni, E.-C. Shin, L. Chiriboga, D. E. Kleiner, and B. Rehermann
Peripheral CD4+CD8+ T cells are differentiated effector memory cells with antiviral functions
Blood,
July 15, 2004;
104(2):
478 - 486.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Zloza, Y. B. Sullivan, E. Connick, A. L. Landay, and L. Al-Harthi
CD8+ T cells that express CD4 on their surface (CD4dimCD8bright T cells) recognize an antigen-specific target, are detected in vivo, and can be productively infected by T-tropic HIV
Blood,
September 15, 2003;
102(6):
2156 - 2164.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Lima, J. Almeida, M. dos Anjos Teixeira, M. d. C. Alguero, A. H. Santos, A. Balanzategui, M. L. Queiros, P. Barcena, A. Izarra, S. Fonseca, et al.
TCR{alpha}{beta}+/CD4+ Large Granular Lymphocytosis: A New Clonal T-Cell Lymphoproliferative Disorder
Am. J. Pathol.,
August 1, 2003;
163(2):
763 - 771.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Jimenez, R. Sacedon, A. Vicente, C. Hernandez-Lopez, A. G. Zapata, and A. Varas
Rat Peripheral CD4+CD8+ T Lymphocytes Are Partially Immunocompetent Thymus-Derived Cells That Undergo Post-Thymic Maturation to Become Functionally Mature CD4+ T Lymphocytes
J. Immunol.,
May 15, 2002;
168(10):
5005 - 5013.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. P. Yang, J. L. Riley, R. G. Carroll, C. H. June, J. Hoxie, B. K. Patterson, Y. Ohshima, R. J. Hodes, and G. Delespesse
Productive Infection of Neonatal CD8+ T Lymphocytes by HIV-1
J. Exp. Med.,
April 6, 1998;
187(7):
1139 - 1144.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H de la Salle, D Hanau, D Fricker, A Urlacher, A Kelly, J Salamero, S. Powis, L Donato, H Bausinger, M Laforet, et al.
Homozygous human TAP peptide transporter mutation in HLA class I deficiency
Science,
July 8, 1994;
265(5169):
237 - 241.
[Abstract]
[PDF]
|
|
|
|
|
