|
|
 Previous Article | Table of Contents | Next Article 
Molecular analysis of VH and VL regions expressed in IgG-bearing chronic
lymphocytic leukemia (CLL): further evidence that CLL is a heterogeneous
group of tumors
SB Ebeling, ME Schutte and T Logtenberg
Department of Immunology, University Hospital, Utrecht, The Netherlands.
We report the heavy (H) and light (L) chain variable (V) region sequences
of cDNAs encoding the Ig receptor of two cases of CD5+ IgG- bearing CLL P87
and P103. In both CLL cases the H chain was encoded by members of the VH3
gene family. The L chain expressed by P87 belonged to the V lambda IV
subgroup, whereas P103 used a member of the V kappa III subgroup. The
VH3.P87 gene differed by only three nucleotides from 38P1, a VH3 gene
previously cloned from a fetal liver cDNA library. Nucleotide sequence
analysis demonstrated that the V kappa III.P103 gene differed by seven
nucleotides from its most homologous germline counterpart, the Humkv325
gene, a highly conserved gene frequently expressed in IgM-bearing CLL. The
nucleotide sequences of VH3.P103 and V lambda IV.P87 could not be reliably
matched with reported germline V genes. The analysis of multiple
independently obtained VH and VL cDNA clones from each tumor showed a lack
of intraclonal diversification. The data show that V regions expressed in
isotype-switched CD5+ CLL may be either in/near germline configuration or
somatically mutated. Furthermore, these tumors, like their IgM-bearing
counterparts, do not seem to undergo intraclonal diversification.
Volume 82,
Issue 5,
pp. 1626-1631,
09/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. N. Potter, C. I. Mockridge, L. Neville, I. Wheatley, M. Schenk, J. Orchard, A. S. Duncombe, G. Packham, and F. K. Stevenson
Structural and Functional Features of the B-Cell Receptor in IgG-Positive Chronic Lymphocytic Leukemia.
Clin. Cancer Res.,
March 15, 2006;
12(6):
1672 - 1679.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Sakai, G. E. Marti, N. Caporaso, S. Pittaluga, J. W. Touchman, F. Fend, and M. Raffeld
Analysis of expressed immunoglobulin heavy chain genes in familial B-CLL
Blood,
February 15, 2000;
95(4):
1413 - 1419.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. Sahota, R. Garand, R. Bataille, A. J. Smith, and F. K. Stevenson
VH Gene Analysis of Clonally Related IgM and IgG From Human Lymphoplasmacytoid B-Cell Tumors With Chronic Lymphocytic Leukemia Features and High Serum Monoclonal IgG
Blood,
January 1, 1998;
91(1):
238 - 243.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Matolcsy, P. Casali, R. G. Nador, Y.-F. Liu, and D. M. Knowles
Molecular Characterization of IgA- and/or IgG-Switched Chronic Lymphocytic Leukemia B Cells
Blood,
March 1, 1997;
89(5):
1732 - 1739.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
