|
|
 Previous Article | Table of Contents | Next Article 
Myeloperoxidase expression in CD34+ normal human hematopoietic cells
H Strobl, M Takimoto, O Majdic, G Fritsch, C Scheinecker, P Hocker and W Knapp
Institute of Immunology, University of Vienna, Austria.
Bone marrow (BM), adult peripheral blood (aPB), and umbilical cord blood
(CB) samples contain small proportions of CD34+ cells that include
virtually all hematopoietic progenitor cells. Myeloperoxidase (MPO) is
considered to be selectively expressed in cells committed to
granulomonocytic differentiation. Using flow cytometry and an antibody
against MPO, we studied at which stage of normal hematopoietic
differentiation CD34+ cells being to express MPO. We consistently observed
a characteristic MPO/CD34 staining pattern and found that 35% +/- 9% of
CD34+ BM cells express MPO. The MPO+ CD34+ subset and the CD33+ CD34+
subset were of similar size and overlapped considerably. MPO+ CD34+ cells
expressed high levels of HLA-D molecules, were weakly CD71/transferrin
receptor positive to negative, were CD45RA+ and lacked the CD45RO isoform
of the leukocyte common antigen. Additionally, MPO+ CD34+ cells were on
average larger in size than MPO- CD34+ cells. Virtually identical
phenotypic features have previously been described for in vitro
colony-forming granulomonocytic progenitor cells. In vitro clonogenic
assays performed with MPO-enriched and MPO-depleted fractions of CD34+ BM
cells performed by us also suggest, but do not formally prove, that at
least a portion of MPO+ CD34+ cells have in vitro cluster (10 to 50
cells/colony) or colony-forming unit granulocyte-macrophage (> or = 50
cells/colony) forming capacity. CD34+ cells from CB and aPB resembled CD34+
BM cells in that considerable proportions of them coexpressed CD33.
However, in contrast to BM, CD34+ cells from CB and aPB samples lacked
significant MPO expression and, in line with this, the majority of them
(CB, 59% +/- 7%; aPB, 66% +/- 5%) coexpressed CD45RO.
Volume 82,
Issue 7,
pp. 2069-2078,
10/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
Y. Chen, P. Haviernik, K. D. Bunting, and Y.-C. Yang
Cited2 is required for normal hematopoiesis in the murine fetal liver
Blood,
October 15, 2007;
110(8):
2889 - 2898.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Roychowdhury, P. M. Vyas, and C. K. Svensson
Formation and Uptake of Arylhydroxylamine-Haptenated Proteins in Human Dendritic Cells
Drug Metab. Dispos.,
April 1, 2007;
35(4):
676 - 681.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. D. Unwin, A. Pierce, R. B. Watson, D. W. Sternberg, and A. D. Whetton
Quantitative Proteomic Analysis Using Isobaric Protein Tags Enables Rapid Comparison of Changes in Transcript and Protein Levels in Transformed Cells
Mol. Cell. Proteomics,
July 1, 2005;
4(7):
924 - 935.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Cimino, L. Elia, M. Mancini, L. Annino, B. Anaclerico, P. Fazi, A. Vitale, G. Specchia, F. Di Raimondo, A. Recchia, et al.
Clinico-biologic features and treatment outcome of adult pro-B-ALL patients enrolled in the GIMEMA 0496 study: absence of the ALL1/AF4 and of the BCR/ABL fusion genes correlates with a significantly better clinical outcome
Blood,
September 15, 2003;
102(6):
2014 - 2020.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Todisco, T. Suzuki, K. Srivannaboon, E. Coustan-Smith, S. C. Raimondi, F. G. Behm, A. Kitanaka, and D. Campana
CD38 ligation inhibits normal and leukemic myelopoiesis
Blood,
January 15, 2000;
95(2):
535 - 542.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Moran, D. Siegel, and D. Ross
A potential mechanism underlying the increased susceptibility of individuals with a polymorphism in NAD(P)H:quinone oxidoreductase 1 (NQO1) to benzene toxicity
PNAS,
July 6, 1999;
96(14):
8150 - 8155.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Scheinecker, K. P. Machold, O. Majdic, P. Hocker, W. Knapp, and J. S. Smolen
Initiation of the Autologous Mixed Lymphocyte Reaction Requires the Expression of Costimulatory Molecules B7-1 and B7-2 on Human Peripheral Blood Dendritic Cells
J. Immunol.,
October 15, 1998;
161(8):
3966 - 3973.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Strobl, C. Scheinecker, E. Riedl, B. Csmarits, C. Bello-Fernandez, W. F. Pickl, O. Majdic, and W. Knapp
Identification of CD68+lin- Peripheral Blood Cells with Dendritic Precursor Characteristics
J. Immunol.,
July 15, 1998;
161(2):
740 - 748.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Oehler, O. Majdic, W. F. Pickl, J. Stockl, E. Riedl, J. Drach, K. Rappersberger, K. Geissler, and W. Knapp
Neutrophil Granulocyte-committed Cells Can Be Driven to Acquire Dendritic Cell Characteristics
J. Exp. Med.,
April 6, 1998;
187(7):
1019 - 1028.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Strobl, C. Bello-Fernandez, E. Riedl, W. F. Pickl, O. Majdic, S. D. Lyman, and W. Knapp
flt3 Ligand in Cooperation With Transforming Growth Factor-beta 1 Potentiates In Vitro Development of Langerhans-Type Dendritic Cells and Allows Single-Cell Dendritic Cell Cluster Formation Under Serum-Free Conditions
Blood,
August 15, 1997;
90(4):
1425 - 1434.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
