|
|
 Previous Article | Table of Contents | Next Article 
Phenotypic and clonal analysis of T lymphocytes in childhood immune
thrombocytopenic purpura
RE Ware and TA Howard
Department of Pediatrics, Duke University Medical Center, Durham, NC 27710.
In an attempt to identify and characterize T-lymphocyte immunoregulatory
abnormalities in immune thrombocytopenic purpura (ITP), we have performed
phenotypic and clonal analysis on peripheral T lymphocytes from 23 children
with ITP. Quantitation of lymphocyte subpopulations showed that children
with acute ITP had higher numbers of CD45RA+ and lower numbers of CD45RO+ T
cells than children with chronic ITP or controls, but these differences may
be age related. Analysis of T-cell receptor variable beta gene usage
identified 2 boys with chronic ITP and elevated numbers of V beta 8+ T
cells. Eight T- cell clones were established (6 CD4+, 4B4+ helper-inducer
lines and 2 CD8+ lines) that showed in vitro proliferation against
allogeneic platelets. The addition of autologous antigen-presenting cells
enhanced the proliferation of six clones, but not for two clones that
coexpressed natural killer (NK) markers. Four of seven positive clones also
had measurable interleukin (IL)-2 secretion following platelet stimulation,
providing further evidence for T-cell reactivity. Our results provide the
first evidence that patients with ITP may have platelet-reactive T
lymphocytes identifiable at the clonal level, supporting the hypothesis
that autoreactive peripheral T lymphocytes may mediate or participate in
the pathogenesis of this disorder.
Volume 82,
Issue 7,
pp. 2137-2142,
10/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
B. Olsson, B. Ridell, L. Carlsson, S. Jacobsson, and H. Wadenvik
Recruitment of T cells into bone marrow of ITP patients possibly due to elevated expression of VLA-4 and CX3CR1
Blood,
August 15, 2008;
112(4):
1078 - 1084.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Stasi, G. Del Poeta, E. Stipa, M. L. Evangelista, M. M. Trawinska, N. Cooper, and S. Amadori
Response to B-cell depleting therapy with rituximab reverts the abnormalities of T-cell subsets in patients with idiopathic thrombocytopenic purpura
Blood,
October 15, 2007;
110(8):
2924 - 2930.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Sukati, H. G. Watson, S. J. Urbaniak, and R. N. Barker
Mapping helper T-cell epitopes on platelet membrane glycoprotein IIIa in chronic autoimmune thrombocytopenic purpura
Blood,
May 15, 2007;
109(10):
4528 - 4538.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Peng, C. Liu, D. Liu, C. Ren, W. Li, Z. Wang, N. Xing, C. Xu, X. Chen, C. Ji, et al.
Effects of B7-blocking agent and/or CsA on induction of platelet-specific T-cell anergy in chronic autoimmune thrombocytopenic purpura
Blood,
April 1, 2003;
101(7):
2721 - 2726.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Mouzaki, M. Theodoropoulou, I. Gianakopoulos, V. Vlaha, M.-C. Kyrtsonis, and A. Maniatis
Expression patterns of Th1 and Th2 cytokine genes in childhood idiopathic thrombocytopenic purpura (ITP) at presentation and their modulation by intravenous immunoglobulin G (IVIg) treatment: their role in prognosis
Blood,
August 13, 2002;
100(5):
1774 - 1779.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. N. Hanft, S. R. Fruchtman, C. V. Pickens, W. F. Rosse, T. A. Howard, and R. E. Ware
Acquired DNA mutations associated with in vivo hydroxyurea exposure
Blood,
June 1, 2000;
95(11):
3589 - 3593.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Nomura, T. Matsuzaki, Y. Ozaki, M. Yamaoka, C. Yoshimura, K. Katsura, G. L. Xie, H. Kagawa, T. Ishida, and S. Fukuhara
Clinical Significance of HLA-DRB1*0410 in Japanese Patients With Idiopathic Thrombocytopenic Purpura
Blood,
May 15, 1998;
91(10):
3616 - 3622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
