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Myofibroblastic stromal cells isolated from human bone marrow induce the
proliferation of both early myeloid and B-lymphoid cells
I Moreau, V Duvert, C Caux, MC Galmiche, P Charbord, J Banchereau and S Saeland
Schering-Plough Laboratory for Immunological Research, Dardilly, France.
Normal human bone marrow stromal cells (BMSC) were isolated from Dexter-
type long-term cultures according to their capacity to adhere to plastic
and to their lack of hematopoietic antigens. The BMSC displayed a
homogeneous appearance and a myofibroblastic phenotype in culture. The
stromal cells (SC) were shown to support the proliferation of purified
CD34+ hematopoietic progenitors and permitted us to maintain myeloid cells
for several weeks in culture. In addition, the BMSC induced the
proliferation of purified CD10+ s mu- fetal BM B-cell precursors (BCP). The
capacity of the BMSC to induce the proliferation of early myeloid cells was
shared by several other human fibroblastic- like cell types. In contrast,
the BMSC were far superior to other adherent cells for induction of BCP
proliferation. This capacity was largely mediated by endogenously produced
interleukin-7 (IL-7), because it could be inhibited by anti-IL-7 antibody.
In line with this finding, addition of IL-7 considerably enhanced BCP
proliferation in cocultures with skin fibroblasts or synoviocytes. Thus,
production of IL-7 appears to be a critical parameter that determines the
ability of fibroblastic- like cells to induce BCP proliferation. Taken
together, our data show that normal human myofibroblastic BMSC induce the
proliferation of both early myeloid and B-lymphoid cells in the absence of
accessory hematopoietic cells. The present system should constitute a model
to study interactions between native human BM myofibroblastic stroma and
various hematopoietic cell subsets.
Volume 82,
Issue 8,
pp. 2396-2405,
10/15/1993
Copyright © 1993 by The American Society of Hematology
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