|
|
 Previous Article | Table of Contents | Next Article 
Competition between plasminogen and tissue plasminogen activator for
cellular binding sites
J Felez, CJ Chanquia, P Fabregas, EF Plow and LA Miles
Institut De Recerca Oncologica, Hospital Duran i Reynals, Autovia de
Castelldefels, L'Hospitalet de Llobregat, Barcelona, Spain.
Cellular receptors for plasminogen and tissue plasminogen activator (t- PA)
regulate plasminogen activation and cell-associated proteolytic activity.
The characteristics of the interactions of both ligands with monocytes and
monocytoid cell lines bear certain similarities, including affinity (kd
approximately 1 mumol/L) capacity and susceptibility to carboxypeptidase
treatment. Therefore, we have undertaken the present study to determine
directly whether t-PA and plasminogen share common binding sites on cells.
We found that recombinant human single-chain t-PA (rt-PA) could inhibit the
binding of 125I-plasminogen to the cells and, conversely, plasminogen could
inhibit 125I-rt-PA binding. This relationship was observed with 9 cell
types, including both adherent cells and cells in suspension. In addition,
under several conditions of cell treatment, plasminogen and t- PA receptor
expression was modulated in parallel. Furthermore, molecules that have been
implicated as candidate plasminogen receptors, gangliosides, and an
alpha-enolase--related molecule, also interacted with t-PA. These results
suggest that at least a component of the binding sites for plasminogen is
shared with t-PA. Occupancy of these sites by either or both ligand(s)
should result in arming the cells with the proteolytic activity of plasmin.
Volume 82,
Issue 8,
pp. 2433-2441,
10/15/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. A. Tanaka, N. S. Key, and J. H. Levy
Blood Coagulation: Hemostasis and Thrombin Regulation
Anesth. Analg.,
May 1, 2009;
108(5):
1433 - 1446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Tanaka, T. Taketomi, F. Szlam, A. Calatzis, and J. H. Levy
Improved Clot Formation by Combined Administration of Activated Factor VII (NovoSeven(R)) and Fibrinogen (Haemocomplettan(R) P)
Anesth. Analg.,
March 1, 2008;
106(3):
732 - 738.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Brownstein, A. B. Deora, A. T. Jacovina, R. Weintraub, M. Gertler, K. M. F. Khan, D. J. Falcone, and K. A. Hajjar
Annexin II mediates plasminogen-dependent matrix invasion by human monocytes: enhanced expression by macrophages
Blood,
January 1, 2004;
103(1):
317 - 324.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. M. Razzaq, R. Bass, D. J. Vines, F. Werner, S. A. Whawell, and V. Ellis
Functional Regulation of Tissue Plasminogen Activator on the Surface of Vascular Smooth Muscle Cells by the Type-II Transmembrane Protein p63 (CKAP4)
J. Biol. Chem.,
October 24, 2003;
278(43):
42679 - 42685.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. J. MacLeod, M. Kwon, N. R. Filipenko, and D. M. Waisman
Phospholipid-associated Annexin A2-S100A10 Heterotetramer and Its Subunits: CHARACTERIZATION OF THE INTERACTION WITH TISSUE PLASMINOGEN ACTIVATOR, PLASMINOGEN, AND PLASMIN
J. Biol. Chem.,
July 3, 2003;
278(28):
25577 - 25584.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Sinniger, R. E. Merton, P. Fabregas, J. Felez, and C. Longstaff
Regulation of Tissue Plasminogen Activator Activity by Cells. DOMAINS RESPONSIBLE FOR BINDING AND MECHANISM OF STIMULATION
J. Biol. Chem.,
April 30, 1999;
274(18):
12414 - 12422.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Ellis and S. A. Whawell
Vascular Smooth Muscle Cells Potentiate Plasmin Generation by Both Urokinase and Tissue Plasminogen Activator-Dependent Mechanisms: Evidence for a Specific Tissue-Type Plasminogen Activator Receptor on These Cells
Blood,
September 15, 1997;
90(6):
2312 - 2322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-O. Kim, E. F. Plow, and L. A. Miles
Regulation of Plasminogen Receptor Expression on Monocytoid Cells by beta 1-Integrin-dependent Cellular Adherence to Extracellular Matrix Proteins
J. Biol. Chem.,
September 27, 1996;
271(39):
23761 - 23767.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
