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A distinct type of sialyl Lewis X antigen defined by a novel monoclonal
antibody is selectively expressed on helper memory T cells
K Ohmori, A Takada, I Ohwaki, N Takahashi, Y Furukawa, M Maeda, M Kiso, A Hasegawa, M Kannagi and R Kannagi
Department of Laboratory Medicine, Kyoto University, School of Medicine,
Japan.
A subset of human helper memory T cells is known to adhere to E- selectin
expressed on cytokine-activated endothelial cells. However, sialyl Lex
antigen, the carbohydrate ligand for E-selectin, has been hardly detectable
on these cells, at least when typical anti-sialyl Lex antibodies were used
for detection. One of the MoAbs (2F3, IgM), which we raised against a
chemically synthesized sialyl Lex glycolipid preparation, is found to react
selectively to CD4+ CD45RObright+ CD45RA- helper memory T cells among
peripheral lymphocytes in healthy individuals. The specificity of the
antibody is in clear contrast to that of the hitherto reported typical
anti-sialyl Lex antibodies FH-6 and SNH-3. These classical anti-sialyl Lex
antibodies were known to react to a subset of natural killer (NK) cells,
but were not reactive with any particular subset of resting peripheral T or
B cells of healthy individuals if the cells were not activated. On the
other hand, the newly generated 2F3 antibody specifically reacted to helper
memory T cells, and did not react to NK cells, B cells, or any T cells
other than helper memory T cells. When tested against the sialyl Lex-active
glycolipid antigen, the reactivity of 2F3 was not significantly different
from that of the classical anti-sialyl Lex antibodies. But when tested
against oligosaccharides prepared from cellular glycoproteins, 2F3 detected
a distinct set of O-linked oligosaccharides, which were not reactive to the
classical anti-sialyl Lex antibodies. Our results suggest that various
molecular species of sialyl Lex antigens are present on carbohydrate side
chains of cellular glycoproteins, and that helper memory T cells express a
distinct type of sialyl Lex antigen that is defined by 2F3 but is not
efficiently detected by other typical anti-sialyl Lex antibodies. Among
cultured lymphocytic leukemia cells, the adult T-cell leukemia (ATL) cells
preferentially expressed the 2F3-defined antigen, and acute lymphocytic
leukemia cells rarely expressed the antigen. The cultured ATL cells
expressing the 2F3-defined antigen showed a clear E-selectin-dependent
adhesion to cytokin-activated endothelial cells, and the 2F3-defined sialyl
Lex antigen served as a ligand for E-selectin as ascertained by the clear
inhibition of adhesion with the 2F3 antibody.(ABSTRACT TRUNCATED AT 400
WORDS)
Volume 82,
Issue 9,
pp. 2797-2805,
11/01/1993
Copyright © 1993 by The American Society of Hematology
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