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Differentiation state and responses to hematopoietic growth factors of
murine myeloid cells transformed by myb
TJ Gonda, EM Macmillan, PV Townsend and AJ Hapel
Hanson Centre for Cancer Research, Adelaide, South Australia.
Murine hematopoietic cells can be transformed in vitro by recombinant
retroviruses that express the myb oncogene, and hematopoietic growth factor
(HGF)-dependent myeloid cell lines can be derived from these transformed
primary cells. In this study, the differentiation state and responses of
myb-transformed hematopoietic cells (MTHCs) have been investigated. We find
that MTHCs exhibit properties of early myeloid progenitors including
synergistic responses to combinations of HGFs and expression of certain
surface markers. As reported previously, MTHCs respond well to
granulocyte-macrophage colony-stimulating factor (GM- CSF) but can also
respond to interleukin-3 (IL-3); the response to the latter factor depends
on the mouse strain from which the cells are derived. Although these single
factors stimulate MTHCs, combinations of these factors with
colony-stimulating factor-1 (CSF-1 or M-CSF) or Steel factor (SLF or SCF)
act synergistically to promote colony formation. The surface markers
expressed by MTHCs include both granulocyte-macrophage lineage specific
antigens Gr-1, 7/4, F4/80, and Mac-1, as well as two antigens found on
early progenitors and stem cells--Thy-1 and Sca-1 (Ly6E). Expression of the
latter markers is often heterogeneous and can be modulated by the growth
factors to which the cells are exposed. Finally, we show that monocytic
differentiation of MTHCs can be induced by exposure to tumor necrosis
factor (TNF alpha). Taken together, these results suggest that MTHCs will
be a useful model for studying HGF/cytokine responses in both proliferation
and differentiation.
Volume 82,
Issue 9,
pp. 2813-2822,
11/01/1993
Copyright © 1993 by The American Society of Hematology
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