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Hematopoietic progenitor cells from patients with adult T-cell leukemia-
lymphoma are not infected with human T-cell leukemia virus type 1
K Nagafuji, M Harada, T Teshima, T Eto, Y Takamatsu, T Okamura, M Murakawa, K Akashi and Y Niho
First Department of Internal Medicine, Faculty of Medicine, Kyushu
University, Fukuoka, Japan.
The in vivo host range of human T-cell leukemia virus type 1 (HTLV-1) has
not been definitively established. To determine if hematopoietic stem cells
from patients with adult T-cell leukemia-lymphoma (ATL) are infected with
HTLV-1, we used a clonogenic progenitor assay followed by the polymerase
chain reaction for the detection of HTLV-1 DNA. In vitro growth
characteristics of myeloid (CFU-GM) and erythroid (BFU-E) progenitor cells
among nonadherent T-cell-depleted bone marrow (BM) mononuclear cells
(NA-T-MNCs) from 10 patients with ATL was not significantly different from
those of HTLV-1-seronegative controls (P = .20); numbers of colonies per 1
x 10(5) NA-T-MNCs were 34.9 +/- 7.6 for CFU-GM and 39.0 +/- 12.5 for BFU-E
in patients with ATL, whereas those were 32.1 +/- 9.5 for CFU-GM and 41.4
+/- 12.7 for BFU-E in normal controls. HTLV-1 DNA was not detected in
individual colonies formed by CD34+ cells from any of the patients.
Similarly HTLV-1 DNA was not detected in 1 x 10(3) CD34+ cells sorted on a
fluorescence-activated cell sorter (FACS) from six patients with ATL
studied. In contrast, HTLV-1 DNA was detected in BM mononuclear cells from
all patients. These observations clearly indicate that hematopoietic
progenitor cells from patients with ATL are normal in their colony-forming
capacity and that CD34+ cells from patients with ATL are not infected with
HTLV-1 in vivo.
Volume 82,
Issue 9,
pp. 2823-2828,
11/01/1993
Copyright © 1993 by The American Society of Hematology
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