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Aged platelets have an impaired response to thrombin as quantitated by
P-selectin expression
J Peng, P Friese, E Heilmann, JN George, SA Burstein and GL Dale
Department of Medicine, University of Oklahoma Health Sciences Center,
Oklahoma City 73190.
After the intravenous infusion of N-hydroxysuccinimido biotin into dogs,
80.6% +/- 9.7% (n = 5) of platelets were covalently labeled with biotin.
The in vivo survival of the biotinylated platelets was monitored by flow
cytometry and was normal as compared with previous reports for dog
platelets. The ability of the biotinylated platelets to be activated was
analyzed by measuring the expression of cell-surface P- selectin after
incubation with graded concentrations of thrombin. When P-selectin
expression was examined 3 hours after labeling, biotinylated platelets were
indistinguishable from the nonlabeled population of platelets, indicating
that biotinylation did not adversely affect the cells. On consecutive days
after biotinylation, the thrombin dose- response curves for biotinylated
and nonbiotinylated platelets were repeated, and as the
biotinylated-platelets aged, they became less responsive to thrombin. On
days 3, 4, and 5, the thrombin EC50 for the aged, biotinylated platelets as
compared with the total population of platelets was 136%, 150%, and 178%,
respectively. Increasing age clearly impairs the reactivity of platelets
towards thrombin as quantitated by the expression of cell-surface
P-selectin.
Volume 83,
Issue 1,
pp. 161-166,
01/01/1994
Copyright © 1994 by The American Society of Hematology
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