|
|
 Previous Article | Table of Contents | Next Article 
In vitro exposure to human immunodeficiency virus type 1 induces apoptotic
cell death of the factor-dependent TF-1 hematopoietic cell line
G Zauli, M Vitale, MC Re, G Furlini, L Zamai, E Falcieri, D Gibellini, G Visani, BR Davis and S Capitani
Institute of Human Anatomy, University of Ferrara, Italy.
In this study, we evaluated the effect of a short-term exposure (2 hours)
to two different lymphocytotropic strains of human immunodeficiency virus
type 1 (HIV-1; HIVIIIB and ICR-3) on the survival of a factor-dependent
CD34+ hematopoietic progenitor cell line (TF-1). At flow cytometry
analysis, a significant (P < .05) increase in the frequency of apoptotic
cell death was observed in HIV-1-treated TF- 1 cells, supplemented with low
doses of either interleukin-3 (IL-3; 0.02 to 1 ng/mL) or
granulocyte-macrophage colony-stimulating factor (GM-CSF; 0.02 to 0.2
ng/mL) with respect to mock-treated cells. On the other hand, higher doses
of both cytokines or combinations of suboptimal concentrations of IL-3 plus
GM-CSF (eg, 0.2, plus 0.2 ng/mL) completely reversed the HIV-1-induced
increase of apoptosis. Remarkably, no signs of productive or latent virus
replication were ever observed in HIV-1-treated TF-1 cells up to 16 days of
liquid culture. In parallel experiments, the in vitro exposure to HIVIIIB
induced a significant and progressive increase of apoptotic death in
purified bone marrow CD34+ cells, seeded in liquid cultures in the presence
of 1 ng/mL IL-3. The HIV-1-induced apoptosis of TF-1 cells was likely
triggered by the simple interaction of HIV-1 envelope glycoprotein gp120
with CD4 receptor, which was expressed at a low level on the surface of
TF-1 cells. In fact, treatment of TF-1 cells with recombinant gp120 plus a
polyclonal anti-gp120 antibody or with anti-CD4 monoclonal antibody plus
rabbit antimouse IgG significantly increased the percentage of apoptotic
death. These data suggest that HIV-1, and perhaps also free gp120 in the
presence of anti-gp120 antibody; could play a direct role in the
pathogenesis of peripheral blood cytopenias in acquired immunodeficiency
syndrome patients by inducing apoptotic death of hematopoietic progenitor
cells without the need of a direct infection.
Volume 83,
Issue 1,
pp. 167-175,
01/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
P. Mirandola, C. Ponti, G. Gobbi, I. Sponzilli, M. Vaccarezza, L. Cocco, G. Zauli, P. Secchiero, F. A. Manzoli, and M. Vitale
Activated human NK and CD8+ T cells express both TNF-related apoptosis-inducing ligand (TRAIL) and TRAIL receptors but are resistant to TRAIL-mediated cytotoxicity
Blood,
October 15, 2004;
104(8):
2418 - 2424.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Secchiero, A. Gonelli, P. Mirandola, E. Melloni, L. Zamai, C. Celeghini, D. Milani, and G. Zauli
Tumor necrosis factor-related apoptosis-inducing ligand induces monocytic maturation of leukemic and normal myeloid precursors through a caspase-dependent pathway
Blood,
September 18, 2002;
100(7):
2421 - 2429.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Secchiero, P. Mirandola, D. Zella, C. Celeghini, A. Gonelli, M. Vitale, S. Capitani, and G. Zauli
Human herpesvirus 7 induces the functional up-regulation of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) coupled to TRAIL-R1 down-modulation in CD4+ T cells
Blood,
October 15, 2001;
98(8):
2474 - 2481.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Secchiero, A. Gonelli, C. Celeghini, P. Mirandola, L. Guidotti, G. Visani, S. Capitani, and G. Zauli
Activation of the nitric oxide synthase pathway represents a key component of tumor necrosis factor-related apoptosis-inducing ligand-mediated cytotoxicity on hematologic malignancies
Blood,
October 1, 2001;
98(7):
2220 - 2228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Colamussi, P. Secchiero, A. Gonelli, M. Marchisio, G. Zauli, and S. Capitani
Stromal derived factor-1{alpha} (SDF-1{alpha}) induces CD4+ T cell apoptosis via the functional up-regulation of the Fas (CD95)/Fas ligand (CD95L) pathway
J. Leukoc. Biol.,
February 1, 2001;
69(2):
263 - 270.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Zauli, D. Gibellini, P. Secchiero, H. Dutartre, D. Olive, S. Capitani, and Y. Collette
Human Immunodeficiency Virus Type 1 Nef Protein Sensitizes CD4+ T Lymphoid Cells to Apoptosis via Functional Upregulation of the CD95/CD95 Ligand Pathway
Blood,
February 1, 1999;
93(3):
1000 - 1010.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Secchiero, L. Bertolaso, L. Casareto, D. Gibellini, M. Vitale, K. Bemis, A. Aleotti, S. Capitani, G. Franchini, R. C. Gallo, et al.
Human Herpesvirus 7 Infection Induces Profound Cell Cycle Perturbations Coupled to Disregulation of cdc2 and Cyclin B and Polyploidization of CD4+ T Cells
Blood,
September 1, 1998;
92(5):
1685 - 1696.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Moses, J. Nelson, and G. C. Bagby Jr
The Influence of Human Immunodeficiency Virus-1 on Hematopoiesis
Blood,
March 1, 1998;
91(5):
1479 - 1495.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Secchiero, L. Flamand, D. Gibellini, E. Falcieri, I. Robuffo, S. Capitani, R. C. Gallo, and G. Zauli
Human Herpesvirus 7 Induces CD4+ T-Cell Death by Two Distinct Mechanisms: Necrotic Lysis in Productively Infected Cells and Apoptosis in Uninfected or Nonproductively Infected Cells
Blood,
December 1, 1997;
90(11):
4502 - 4512.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. N. Economides, J. V. Ravetch, G. D. Yancopoulos, and N. Stahl
Designer Cytokines: Targeting Actions to Cells of Choice
Science,
November 24, 1995;
270(5240):
1351 - 1353.
[Abstract]
[PDF]
|
|
|
|
|
