|
|
 Previous Article | Table of Contents | Next Article 
Molecular cloning of the breakpoint for 3q27 translocation in B-cell
lymphomas and leukemias
T Miki, N Kawamata, A Arai, K Ohashi, Y Nakamura, A Kato, S Hirosawa and N Aoki
First Department of Internal Medicine, Tokyo Medical and Dental University,
Japan.
Reciprocal exchanges between chromosomal region 3q27 and three loci of the
Ig genes have been reported in cases of B-cell type non-Hodgkin's lymphoma.
We have cloned a region containing a breakpoint junction of 3q27 from a
cell line established from a patient with Burkitt's lymphoma carrying
t(3;22)(q27;q11). The region cloned was shown to contain an Ig lambda light
chain gene fused to a gene on chromosome 3q27. This finding was
subsequently confirmed by fluorescence in situ hybridization. Extra
nucleotides were present at the joining site. The heptamer-like and
nonamer-like sequences separated by an intervening 24 bp were present in
the region corresponding to the breakpoint of 3q27, suggesting that a
misrecombination in Ig gene rearrangement may be involved in the
translocation. Southern blot analysis with a 3q27- specific probe showed
rearrangements in three additional patients with B-cell malignancies with
the t(3;14)(q27;q32). The breakpoints of all four cases clustered within a
limited 3-kb region on chromosome 3q27. The region of 3q27 involved in the
translocation was designated as the BCL5 locus. The transcripts from the
BCL5 locus were detected in normal tissues and hematopoietic cell lines,
and the increased expression of transcript of aberrant size was detected in
the established cell line carrying t(3;22). These observations suggest that
a gene located at 3q27 is involved in the translocation and that its
deregulation plays a role in the malignant transformation of B cells.
Volume 83,
Issue 1,
pp. 217-222,
01/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Morishima, A. Marui, T. Shimamoto, Y. Saji, K. Tambara, T. Nishina, and M. Komeda
Successful Aortic Valve Replacement for Heyde Syndrome With Confirmed Hematologic Recovery
Ann. Thorac. Surg.,
January 1, 2007;
83(1):
287 - 288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Katzenberger, G. Ott, T. Klein, J. Kalla, H. K. Muller-Hermelink, and M. M. Ott
Cytogenetic Alterations Affecting BCL6 Are Predominantly Found in Follicular Lymphomas Grade 3B with a Diffuse Large B-Cell Component
Am. J. Pathol.,
August 1, 2004;
165(2):
481 - 490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Smith and M. Sigvardsson
The roles of transcription factors in B lymphocyte commitment, development, and transformation
J. Leukoc. Biol.,
June 1, 2004;
75(6):
973 - 981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Wlodarska, P. Nooyen, B. Maes, J. I. Martin-Subero, R. Siebert, P. Pauwels, C. De Wolf-Peeters, and A. Hagemeijer
Frequent occurrence of BCL6 rearrangements in nodular lymphocyte predominance Hodgkin lymphoma but not in classical Hodgkin lymphoma
Blood,
January 15, 2003;
101(2):
706 - 710.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Wang, Z. Li, A. Naganuma, and B. H. Ye
Negative autoregulation of BCL-6 is bypassed by genetic alterations in diffuse large B cell lymphomas
PNAS,
November 12, 2002;
99(23):
15018 - 15023.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. D. Huynh, W. Fischle, E. Verdin, and V. J. Bardwell
BCoR, a novel corepressor involved in BCL-6 repression
Genes & Dev.,
July 15, 2000;
14(14):
1810 - 1823.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. E. Hoatlin, Y. Zhi, H. Ball, K. Silvey, A. Melnick, S. Stone, S. Arai, N. Hawe, G. Owen, A. Zelent, et al.
A Novel BTB/POZ Transcriptional Repressor Protein Interacts With the Fanconi Anemia Group C Protein and PLZF
Blood,
December 1, 1999;
94(11):
3737 - 3747.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yoshida, T. Fukuda, M. Hatano, H. Koseki, S.-i. Okabe, K. Ishibashi, S. Kojima, M. Arima, I. Komuro, G. Ishii, et al.
The role of Bcl6 in mature cardiac myocytes
Cardiovasc Res,
June 1, 1999;
42(3):
670 - 679.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Fukuda, T. Yoshida, S. Okada, M. Hatano, T. Miki, K. Ishibashi, S. Okabe, H. Koseki, S. Hirosawa, M. Taniguchi, et al.
Disruption of the Bcl6 Gene Results in an Impaired Germinal Center Formation
J. Exp. Med.,
August 4, 1997;
186(3):
439 - 448.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Nishida, A. Tamura, N. Nakazawa, Y. Ueda, T. Abe, F. Matsuda, K. Kashima, and M. Taniwaki
The Ig Heavy Chain Gene Is Frequently Involved in Chromosomal Translocations in Multiple Myeloma and Plasma Cell Leukemia as Detected by In Situ Hybridization
Blood,
July 15, 1997;
90(2):
526 - 534.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Gaidano, A. Carbone, C. Pastore, D. Capello, A. Migliazza, A. Gloghini, S. Roncella, M. Ferrarini, G. Saglio, and R. Dalla-Favera
Frequent Mutation of the 5' Noncoding Region of the BCL-6 Gene in Acquired Immunodeficiency Syndrome-Related Non-Hodgkin's Lymphomas
Blood,
May 15, 1997;
89(10):
3755 - 3762.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
