|
|
 Previous Article | Table of Contents | Next Article 
Platelet adhesion to collagen in individuals lacking glycoprotein IV
L McKeown, M Vail, S Williams, W Kramer, K Hansmann and H Gralnick
Hematology Service, National Institutes of Health, Bethesda, MD 20892.
The Naka isoantigen is expressed on glycoprotein (GP) IV (CD36), a platelet
membrane GP that has been identified as having a role in platelet
interactions with collagen and thrombospondin and in binding Plasmodium
falciparum-infected erythrocytes to endothelial cells and melanoma cells.
We have studied normal platelets and Naka- platelets from two Japanese
donors that have 1% of GPIV by concentration- dependent antibody binding
and flow cytometry. We studied the adherence of normal and Naka- platelets
to types I, III, and IV collagen in static and to type I collagen in
flowing systems at high shear force. We have also studied aggregation of
normal and Naka- platelets to type I collagen. Naka- platelets showed
normal or increased aggregation to type I collagen and normal adhesion to
types I, III, and IV collagen in the presence of Mg++ or EDTA. Platelet
aggregation and adhesion were inhibited by the anti-alpha 2 beta 1 antibody
176D7 to the same extent in Naka- as in normal platelets. We also studied
endogenous thrombospondin surface expression and found that
thrombin-stimulated Naka- platelets expressed the same amount of
thrombospondin as did normal platelets. From our studies with Naka-
platelets, we cannot identify a definitive role for GPIV in platelet
aggregation, in adhesion to types I, III, and IV collagen, or in endogenous
thrombospondin binding to platelets.
Volume 83,
Issue 10,
pp. 2866-2871,
05/15/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Nakata, Y. Nakagawa, M. Nishida, S. Nozaki, J.-i. Miyagawa, T. Nakagawa, R. Tamura, K. Matsumoto, K. Kameda-Takemura, S. Yamashita, et al.
CD36, a Novel Receptor for Oxidized Low-Density Lipoproteins, Is Highly Expressed on Lipid-Laden Macrophages in Human Atherosclerotic Aorta
Arterioscler Thromb Vasc Biol,
May 1, 1999;
19(5):
1333 - 1339.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Nakamura, J.-i. Kambayashi, M. Okuma, and N. N. Tandon
Activation of the GP IIb-IIIa Complex Induced by Platelet Adhesion to Collagen Is Mediated by Both {alpha}2{beta}1 Integrin and GP VI
J. Biol. Chem.,
April 23, 1999;
274(17):
11897 - 11903.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Nakamura, G. A. Jamieson, M. Okuma, J.-i. Kambayashi, and N. N. Tandon
Platelet Adhesion to Native Type I Collagen Fibrils. ROLE OF GPVI IN DIVALENT CATION-DEPENDENT AND -INDEPENDENT ADHESION AND THROMBOXANE A2 GENERATION
J. Biol. Chem.,
February 20, 1998;
273(8):
4338 - 4344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Kehrel, S. Wierwille, K. J. Clemetson, O. Anders, M. Steiner, C. Graham Knight, R. W. Farndale, M. Okuma, and M. J. Barnes
Glycoprotein VI Is a Major Collagen Receptor for Platelet Activation: It Recognizes the Platelet-Activating Quaternary Structure of Collagen, Whereas CD36, Glycoprotein IIb/IIIa, and von Willebrand Factor Do Not
Blood,
January 15, 1998;
91(2):
491 - 499.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Polgar, J. M. Clemetson, B. E. Kehrel, M. Wiedemann, E. M. Magnenat, T. N. C. Wells, and K. J. Clemetson
Platelet Activation and Signal Transduction by Convulxin, a C-type Lectin from Crotalus durissus terrificus (Tropical Rattlesnake) Venom via the p62/GPVI Collagen Receptor
J. Biol. Chem.,
May 23, 1997;
272(21):
13576 - 13583.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Keely and L. V. Parise
The alpha 2beta 1 Integrin Is a Necessary Co-receptor for Collagen-induced Activation of Syk and the Subsequent Phosphorylation of Phospholipase Cgamma 2 in Platelets
J. Biol. Chem.,
October 25, 1996;
271(43):
26668 - 26676.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Kundu, E. J. Heilmann, R. Sio, C. Garcia, and R. A. Ostgaard
Characterization of an In Vitro Platelet Function Analyzer, PFA-100TM
Clinical and Applied Thrombosis/Hemostasis,
October 1, 1996;
2(4):
241 - 249.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Gibbins, J. Asselin, R. Farndale, M. Barnes, C.-L. Law, and S. P. Watson
Tyrosine Phosphorylation of the Fc Receptor gamma -Chain in Collagen-stimulated Platelets
J. Biol. Chem.,
July 26, 1996;
271(30):
18095 - 18099.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Diaz-Ricart, N. N. Tandon, G. Gomez-Ortiz, M. Carretero, G. Escolar, A. Ordinas, and G.A. Jamieson
Antibodies to CD36 (GPIV) Inhibit Platelet Adhesion to Subendothelial Surfaces Under Flow Conditions
Arterioscler Thromb Vasc Biol,
July 1, 1996;
16(7):
883 - 888.
[Abstract]
[Full Text]
|
|
|
|
|
