Effect of granulocyte-macrophage colony-stimulating factor on sepsis-
induced organ injury in rats
H Toda, A Murata, Y Oka, K Uda, N Tanaka, I Ohashi, T Mori and N Matsuura
Department of Surgery II, Osaka University Medical School, Japan.
The administration of granulocyte-macrophage colony-stimulating factor
(GM-CSF) to patients with severe active infections has been questioned
because activation of neutrophils may cause tissue injury. To identify the
effect of GM-CSF administration on severe sepsis, we examined the survival
rate and pathologic changes in vital organs using the rat lethal sepsis
model. Rats received 20 micrograms of recombinant murine GM-CSF (rmGM-CSF)
3 hours after the onset of peritonitis induced by cecal ligation and
puncture. After 48 hours, the survival rate did not improve, and earlier
deaths than in the control group were observed. In addition, the inhibition
of early leuko-sequestration in the peritoneal cavity was seen in animals
treated with GM-CSF. These results suggested that the administration of
rmGM-CSF after the onset of sepsis was not beneficial; thus, we concluded
that care should be taken in the clinical use of GM-CSF in severe
infection.
Volume 83,
Issue 10,
pp. 2893-2898,
05/15/1994
Copyright © 1994 by The American Society of Hematology
