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Granulocyte-macrophage colony-stimulating factor, interleukin-3 (IL-3), and
IL-5 greatly enhance the interaction of human eosinophils with opsonized
particles by changing the affinity of complement receptor type 3
M Blom, AT Tool, PT Kok, L Koenderman, D Roos and AJ Verhoeven
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service,
University of Amsterdam, The Netherlands.
Eosinophil functions can be modulated by several cytokines such as
granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin- 3
(IL-3), and IL-5. We have investigated the modulatory role of these
cytokines on the interaction of human eosinophils with opsonized particles
(serum-treated zymosan [STZ]). Addition of STZ to eosinophils isolated from
the peripheral blood of normal human donors resulted in an interaction of
the STZ particles with only 15% to 25% of the cells. Treatment of the
eosinophils with GM-CSF, IL-3, or IL-5 strongly enhanced both the rate of
particle binding and the percentage of eosinophils binding STZ. The effect
of the cytokines is most likely mediated by a change in affinity of the
complement receptor type 3 (CR3) on the eosinophils for the complement
fragment iC3b on the STZ particles. This is indicated by the observation
that (1) the effect of the cytokines on STZ binding was prevented by a
monoclonal antibody against the iC3b-binding site on CR3 and (2) the
enhanced binding was already apparent before upregulation of CR3 on the
cell surface was observed. In a previous study, similar results were
obtained with platelet-activating factor (PAF)-primed eosinophils. Because
we found that the cytokines strongly enhanced the STZ-induced PAF
synthesis, we investigated the role of both released PAF and
cell-associated PAF in the priming phenomenon by the cytokines. Cytokine
priming appeared to be largely independent of the synthesis of PAF.
Volume 83,
Issue 10,
pp. 2978-2984,
05/15/1994
Copyright © 1994 by The American Society of Hematology
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