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Platelet adhesion to collagen and endothelial cell matrix under flow
conditions is not dependent on platelet glycoprotein IV
EU Saelman, B Kehrel, KM Hese, PG de Groot, JJ Sixma and HK Nieuwenhuis
Department of Haematology, University Hospital Utrecht, The Netherlands.
Platelet membrane glycoprotein IV (GPIV) is a cell-surface glycoprotein
that has been proposed as a receptor for collagen. Recently, it has been
shown that platelets with the Naka-negative phenotype lack GPIV on their
surface, whereas donors with this phenotype are healthy and do not suffer
from hematologic disorders. In this study, we compared Naka- negative
platelets with normal platelets in adhesion to collagen types I, III, IV,
and V and the extracellular matrix of endothelial cells (ECM) under static
and flow conditions. No differences in platelet adhesion and subsequent
aggregate formation on the collagens types I, III, and IV were observed
under static and flow conditions. Adhesion of both homozygous and
heterozygous Naka-negative platelets to collagen type V was strongly
reduced under static conditions. Collagen type V was not adhesive under
flow conditions. No difference in platelet adhesion to ECM was observed,
which suggests that GPIV is not important in adhesion to subendothelium,
for which ECM may serve as a model. These results indicate that GPIV is not
a functional receptor for collagen under flow conditions.
Volume 83,
Issue 11,
pp. 3240-3244,
06/01/1994
Copyright © 1994 by The American Society of Hematology
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