|
|
 Previous Article | Table of Contents | Next Article 
Establishment and characterization of cloned human thymic epithelial cell
lines. Analysis of adhesion molecule expression and cytokine production
E Fernandez, A Vicente, A Zapata, B Brera, JJ Lozano, C Martinez and ML Toribio
Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid,
Spain.
The thymic stromal microenvironment is required for the generation of
immunocompetent T lymphocytes. However, the different thymic stromal cell
types have not been fully characterized and their roles regarding T-cell
development are not completely understood. To address the phenotypic
characteristics of the epithelial component of the human thymic
microenvironment as well as its functional involvement in T-cell
development, we have established cloned thymic epithelial cell (TEC) lines
from fetal and postnatal human thymuses by an explant technique, repeated
subculture, and limiting dilution cloning. These cloned TEC lines were
shown to be derived from cortical epithelium and to express a number of
cell-surface molecules including CD40, major histocompatibility complex
(MHC) HLA-ABC and HLA-DR antigens, homing- associated cell-adhesion
molecule (H-CAM), intercellular adhesion molecule-1 (ICAM-1), leukocyte
function-associated antigen 3 (LFA-3), and beta 1 subfamily integrins.
Finally, both postnatal and fetal TEC clones were shown to produce
interleukin-1 alpha (IL-1 alpha), IL-6, and IL-7. These well-defined cloned
TEC lines may provide useful tools for the study of TEC biology and for the
understanding of the precise role played by TEC in human T-cell
development.
Volume 83,
Issue 11,
pp. 3245-3254,
06/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. S. Koh, A. J. Kuo, S. Y. Park, P. Cheung, J. Abramson, D. Bua, D. Carney, S. E. Shoelson, O. Gozani, R. E. Kingston, et al.
Aire employs a histone-binding module to mediate immunological tolerance, linking chromatin regulation with organ-specific autoimmunity
PNAS,
October 14, 2008;
105(41):
15878 - 15883.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Herschke, S. Plumet, T. Duhen, O. Azocar, J. Druelle, D. Laine, T. F. Wild, C. Rabourdin-Combe, D. Gerlier, and H. Valentin
Cell-Cell Fusion Induced by Measles Virus Amplifies the Type I Interferon Response
J. Virol.,
December 1, 2007;
81(23):
12859 - 12871.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Lepelletier, S. Smaniotto, R. Hadj-Slimane, D. M. S. Villa-Verde, A. C. Nogueira, M. Dardenne, O. Hermine, and W. Savino
Control of human thymocyte migration by Neuropilin-1/Semaphorin-3A-mediated interactions
PNAS,
March 27, 2007;
104(13):
5545 - 5550.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ichimiya and T. Kojima
Cellular Networks of Human Thymic Medullary Stromas Coordinated by p53-Related Transcription Factors
J. Histochem. Cytochem.,
November 1, 2006;
54(11):
1277 - 1289.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kikuchi, S. Ichimiya, T. Kojima, L. Crisa, S. Koshiba, A. Tonooka, N. Kondo, P. T. van der Saag, S. Yokoyama, and N. Sato
Expression profiles and functional implications of p53-like transcription factors in thymic epithelial cell subtypes
Int. Immunol.,
June 1, 2004;
16(6):
831 - 841.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Sacedon, A. Varas, C. Hernandez-Lopez, C. Gutierrez-deFrias, T. Crompton, A. G. Zapata, and A. Vicente
Expression of Hedgehog Proteins in the Human Thymus
J. Histochem. Cytochem.,
November 1, 2003;
51(11):
1557 - 1566.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Laine, M.-C. Trescol-Biemont, S. Longhi, G. Libeau, J. C. Marie, P.-O. Vidalain, O. Azocar, A. Diallo, B. Canard, C. Rabourdin-Combe, et al.
Measles Virus (MV) Nucleoprotein Binds to a Novel Cell Surface Receptor Distinct from Fc{gamma}RII via Its C-Terminal Domain: Role in MV-Induced Immunosuppression
J. Virol.,
November 1, 2003;
77(21):
11332 - 11346.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Bania, E. Gatti, H. Lelouard, A. David, F. Cappello, E. Weber, V. Camosseto, and P. Pierre
Human cathepsin S, but not cathepsin L, degrades efficiently MHC class II-associated invariant chain in nonprofessional APCs
PNAS,
May 27, 2003;
100(11):
6664 - 6669.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P.-O. Vidalain, D. Laine, Y. Zaffran, O. Azocar, C. Servet-Delprat, T. F. Wild, C. Rabourdin-Combe, and H. Valentin
Interferons Mediate Terminal Differentiation of Human Cortical Thymic Epithelial Cells
J. Virol.,
June 5, 2002;
76(13):
6415 - 6424.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ichimiya, T. Kojima, H. Momota, N. Kondo, T. Ozaki, A. Nakagawara, M. L. Toribio, M. Imamura, and N. Sato
p73 Is Expressed in Human Thymic Epithelial Cells
J. Histochem. Cytochem.,
April 1, 2002;
50(4):
455 - 462.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Valentin, O. Azocar, B. Horvat, R. Williems, R. Garrone, A. Evlashev, M. L. Toribio, and C. Rabourdin-Combe
Measles Virus Infection Induces Terminal Differentiation of Human Thymic Epithelial Cells
J. Virol.,
March 1, 1999;
73(3):
2212 - 2221.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
V. de Mello-Coelho, M.-C. Gagnerault, J.-C. Souberbielle, C. J. Strasburger, W. Savino, M. Dardenne, and M.-C. Postel-Vinay
Growth Hormone and Its Receptor Are Expressed in Human Thymic Cells
Endocrinology,
September 1, 1998;
139(9):
3837 - 3842.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Romagnani, F. Annunziato, R. Manetti, C. Mavilia, L. Lasagni, C. Manuelli, G. B. Vannelli, V. Vanini, E. Maggi, C. Pupilli, et al.
High CD30 Ligand Expression by Epithelial Cells and Hassal's Corpuscles in the Medulla of Human Thymus
Blood,
May 1, 1998;
91(9):
3323 - 3332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. R. Salomon, L. Crisa, C. F. Mojcik, J. K. Ishii, G. Klier, and E. M. Shevach
Vascular Cell Adhesion Molecule-1 Is Expressed by Cortical Thymic Epithelial Cells and Mediates Thymocyte Adhesion. Implications for the Function of alpha 4beta 1 (VLA4) Integrin in T-Cell Development
Blood,
April 1, 1997;
89(7):
2461 - 2471.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
