Genotypical classification of neutrophil Fc gamma receptor III by
polymerase chain reaction-single-strand conformation polymorphism
T Satoh, M Kobayashi, M Kaneda, M Tanihiro, K Okada and K Ueda
Department of Pediatrics, Hiroshima University School of Medicine, Japan.
We classified the genotype of neutrophil Fc gamma receptor III (FcRIII)
(CD16) with a new method. Genomic DNA from mononuclear cells of 39
unrelated healthy donors (13 NA1/NA1, 13 NA2/NA2, and 13 NA1/NA2 typed
serologically) were subjected to polymerase chain reaction (PCR) to amplify
the polymorphic third exon of the FcRIII genes. The PCR products were heat
denatured, electrophoresed, and visualized by silver staining. Allelic
differences were detected by distinctive electrophoretic patterns of each
single strand, depending on their sequence specific conformations
(single-strand conformation polymorphism [SSCP]). The genotypes of
neutrophil FcRIII determined by this method were consistent with the
phenotypes of NA antigens determined by serologic examinations. These
results indicate that the PCR-SSCP system is a very useful tool for
genotyping of the neutrophil FcRIII.
Volume 83,
Issue 11,
pp. 3312-3315,
06/01/1994
Copyright © 1994 by The American Society of Hematology
