Paternal origin of the rearranged major breakpoint cluster region in
chronic myeloid leukemia
CE Litz and CM Copenhaver
Department of Laboratory Medicine and Pathology, University of Minnesota
Hospital and Clinic, Minneapolis 55455.
The Philadelphia chromosome, t(9;22), is present in virtually all cases of
chronic myeloid leukemia (CML). It has previously been shown by cytogenetic
studies that the rearranged chromosome 22 in patients with CML is
exclusively maternal in origin. To address this issue at a molecular level,
the major breakpoint cluster region (M-bcr) on chromosome 22 was examined
using Southern blot assays and M-bcr Pvu II and Mae II restriction site
polymorphisms in three CML patients. In all three cases, the rearranged
allele was paternal in origin. These results indicate that the paternally
derived M-bcr allele may also be involved in the M-bcr rearrangement.
Volume 83,
Issue 12,
pp. 3445-3448,
06/15/1994
Copyright © 1994 by The American Society of Hematology
