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C-kit gene is expressed by skin mast cells in embryos but not in puppies of
Wsh/Wsh mice: age-dependent abolishment of c-kit gene expression
M Yamazaki, T Tsujimura, E Morii, K Isozaki, H Onoue, S Nomura and Y Kitamura
Department of Pathology, Osaka University Medical School, Japan.
The Wsh is a mutant allele at the W (c-kit) locus of mice, but no
significant abnormalities are found at the coding region of the Wsh allele.
Since cultured mast cells derived from the spleen of Wsh/Wsh mice do not
express messenger RNA (mRNA) of c-kit, we studied the interrelation between
the number of mast cells and the magnitude of c- kit mRNA expression in the
skin of Wsh/Wsh mice of various ages. The number of mast cells in the skin
of Wsh/Wsh embryos of 18 days postcoitum (pc) was approximately 40% that of
normal control (+/+) embryos, but the number of mast cells decreased
exponentially after birth; the number dropped to 0.6% that of +/+ mice at
day 150 after birth. A weak but apparent signal of c-kit mRNA was
detectable in the skin of 18-day pc Wsh/Wsh embryos by RNase protection
assay but not in the skin of 5-day-old Wsh/Wsh mice. The number of c-kit
protein- containing cells was significantly greater in the skin of 18-day
pc Wsh/Wsh embryos than in the skin of 5-day-old Wsh/Wsh mice. The
abolishment of c-kit mRNA expression appeared to be specific, because the
expression of mast cell carboxypeptidase A mRNA but not of c-kit mRNA was
detectable by in situ hybridization in skin mast cells of 5- day-old
Wsh/Wsh mice. Taken together, the expression of c-kit mRNA was abolished
first, then the content of c-kit protein dropped to undetectable levels,
and then the disappearance of Wsh/Wsh mast cells themselves followed.
Volume 83,
Issue 12,
pp. 3509-3516,
06/15/1994
Copyright © 1994 by The American Society of Hematology
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