|
|
 Previous Article | Table of Contents | Next Article 
Growth inhibitory and agonistic signals of interleukin-7 (IL-7) can be
mediated through the CDw127 IL-7 receptor
D Pandrau-Garcia, B de Saint-Vis, S Saeland, N Renard, S Ho, I Moreau, J Banchereau and JP Galizzi
Schering-Plough Laboratory for Immunological Research, Dardilly, France.
The present study was aimed at identifying surface-membrane molecules
involved in the regulation of human B-cell ontogeny. For this purpose,
murine monoclonal antibodies (MoAbs) were generated against Pre-Alp, a
pre-B acute lymphoblastic leukemia (ALL) cell line, and MoAb R34.34 was
selected for further characterization. R34.34 recognized a molecule
expressed on normal B-cell precursors (BCP) but not on mature B cells. The
antibody also reacted with T lymphocytes, a subpopulation of monocytes from
peripheral blood, and a subset of CD34+ cells. Immunoprecipitation analysis
indicated that R34.34 recognizes an 80-kD molecular weight antigen.
Antibody R34.34 was further found to be directed against an epitope
interfering with binding of interleukin-7 (IL-7) to Pre-Alp cells.
Expression cloning from a Pre-Alp cDNA library showed that R34.34 antigen
is CDw127, the 75- to 80-kD IL-7 receptor. Proliferation of the B-lineage
ALL cell lines Reh and Mieliki was inhibited by IL-7, and this effect was
specifically reverted by MoAb R34.34. In addition, antibody R34.34
specifically inhibited IL-7- dependent proliferation of normal BCP, Pre-Alp
cells, and peripheral T cells. These results imply that both inhibitory and
proliferative effects of IL-7 can be mediated through the same receptor on
various lineages. R34.34 antibody should be important for the analysis of
signal transduction through CDw127.
Volume 83,
Issue 12,
pp. 3613-3619,
06/15/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Higgins, J. A. Metcalf, R. A. Stevens, M. Baseler, M. C. Nason, H. C. Lane, and I. Sereti
Effects of Lymphocyte Isolation and Timing of Processing on Detection of CD127 Expression on T Cells in Human Immunodeficiency Virus-Infected Patients
Clin. Vaccine Immunol.,
January 1, 2005;
12(1):
228 - 230.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. F. A. Weekx, H. W. Snoeck, F. Offner, M. De Smedt, D. R. Van Bockstaele, G. Nijs, M. Lenjou, A. Moulijn, I. Rodrigus, Z. N. Berneman, et al.
Generation of T cells from adult human hematopoietic stem cells and progenitors in a fetal thymic organ culture system: stimulation by tumor necrosis factor-alpha
Blood,
May 1, 2000;
95(9):
2806 - 2812.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. H. Ryan, B. L. Nuccie, I. Ritterman, J. L. Liesveld, C. N. Abboud, and R. A. Insel
Expression of Interleukin-7 Receptor by Lineage-Negative Human Bone Marrow Progenitors With Enhanced Lymphoid Proliferative Potential and B-Lineage Differentiation Capacity
Blood,
February 1, 1997;
89(3):
929 - 940.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
