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Native associations of early hematopoietic stem cells and stromal cells
isolated in bone marrow cell aggregates
PE Funk, PW Kincade and PL Witte
Department of Microbiology, Loyola University Medical Center, Maywood, IL
60153.
In suspensions of murine bone marrow, many stromal cells are tightly
entwined with hematopoietic cells. These cellular aggregations appear to
exist normally within the marrow. Previous studies showed that lymphocytes
and stem cells adhered to stromal cells via vascular cell adhesion molecule
1 (VCAM1). Injection of anti-VCAM1 antibody into mice disrupts the
aggregates, showing the importance of VCAM1 in the adhesion between stromal
cells and hematopoietic cells in vivo. Early hematopoietic stem cells were
shown to be enriched in aggregates by using a limiting-dilution culture
assay. Myeloid progenitors responsive to WEHI-3CM in combination with stem
cell factor (c-kit ligand) and B220- B-cell progenitors responsive to
insulin-like growth factor-1 in combination with interleukin-7 are not
enriched. We propose a scheme of stromal cell-hematopoietic cell
interactions based on the cell types selectively retained within the
aggregates. The existence of these aggregates as native elements of bone
marrow organization presents a novel means to study in vivo stem
cell-stromal cell interaction.
Volume 83,
Issue 2,
pp. 361-369,
01/15/1994
Copyright © 1994 by The American Society of Hematology
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