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Human cytomegalovirus alters interleukin-6 production by endothelial cells
GD Almeida, CD Porada, S St Jeor and JL Ascensao
University of Nevada, School of Medicine, VA Medical Center, Reno 89520.
In an effort to study whether human cytomegalovirus (HCMV) can disrupt the
balanced cytokine network that controls human hematopoiesis, we
investigated the ability of a laboratory strain HCMV (AD169) to alter the
production of interleukin-6 (IL-6) by cultured endothelial cells (HUVECs).
ECs are important components of human bone marrow stroma and produce
factors that stimulate the proliferation and differentiation of human
hematopoietic progenitors. HCMV was able to greatly increase production of
both mRNA and protein for IL-6 in unprimed HUVECs. When we discriminated
between viral pellet and cleared viral supernatants, the supernatants
induced an increase in mRNA at 30 minutes and protein by 2 hours, whereas
an increase in IL-6 caused by virus itself did not become evident until 12
hours. The possibility that IL-6 induction was simply caused by the
presence in the viral stock of endotoxin, IL-1 alpha, IL-1 beta, tumor
necrosis factor alpha, or IL-4, all known inducers of IL-6 in HUVECs, was
ruled out by the addition of polymyxin B and appropriate neutralizing
antibodies. These findings show that HCMV is capable of directly and
indirectly modulating the production by HUVECs of IL-6, one of the
cytokines involved in the process of hematopoiesis.
Volume 83,
Issue 2,
pp. 370-376,
01/15/1994
Copyright © 1994 by The American Society of Hematology
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