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Activation of beta 1 integrin fibronectin receptors on HL60 cells after
granulocytic differentiation
JF Bohnsack and J Chang
Department of Pediatrics, University of Utah School of Medicine, Salt Lake
City 84132.
Phorbol esters upregulate the functional affinity of beta 1 integrin
receptors for fibronectin on human neutrophils and other leukocytes. We
investigated the ability of phorbol myristate acetate (PMA) to stimulate
the human promyelocytic cell line HL-60 to adhere to fibronectin, either in
its undifferentiated state (HL60) or after dimethylsulfoxide-induced
differentiation along the granulocytic pathway (dHL60). PMA stimulated
little adherence of undifferentiated HL60 to fibronectin or to the 120-kD
chymotryptic cell-binding domain (CBD) of fibronectin. In contrast, PMA
stimulated dHL60 cells to rapidly adhere to both fibronectin- and to
CBD-coated plastic. PMA- stimulated dHL60 adherence to fibronectin was
largely mediated by both alpha 4 beta 1 and alpha 5 beta 1, whereas
PMA-stimulated dHL60 adherence to CBD was largely mediated by alpha 5 beta
1. There was little contribution from beta 2 integrins to PMA-stimulated
dHL60 adherence to fibronectin or CBD. The inability of undifferentiated
HL60 to adhere to fibronectin and CBD did not result from lack of
expression of alpha 4 beta 1 or alpha 5 beta 1 because HL60 and dHL60
express similar amounts of both alpha 4 beta 1 and alpha 5 beta 1 on their
surface. In addition, 1 mmol/L Mn2+ induced similar amounts of alpha 5 beta
1-dependent adherence of both HL60 and dHL60, showing that alpha 5 beta 1
on undifferentiated HL60 is capable of binding to its ligand. These data
suggest that activation of protein kinase C cannot functionally upregulate
these beta 1 integrins on undifferentiated HL60 cells. The development of
PMA-stimulated beta 1-dependent adherence after granulocytic
differentiation of HL60 cells suggests that the differentiated HL60 cell is
a useful model for investigating functional coupling of protein kinase C to
beta 1 integrin in myeloid cells.
Volume 83,
Issue 2,
pp. 543-552,
01/15/1994
Copyright © 1994 by The American Society of Hematology
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