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CD44 mediates hyaluronan binding by human myeloid KG1A and KG1 cells
K Morimoto, E Robin, MC Le Bousse-Kerdiles, Y Li, D Clay, C Jasmin and F Smadja- Joffe
Unite d'Oncogenese Appliquee, Inserm U268, Hopital Paul Brousse, Villejuif,
France.
Hyaluronan-binding function of the CD44 molecule has not been so far
detected in myeloid cells. To study pure populations of primitive myeloid
cells, we investigated the hyaluronan-binding function of the CD44 molecule
from three myeloid cell lines: KG1a, KG1, and HL60. Both KG1a and KG1 cells
express the CD34 antigen characteristic of the hematopoietic stem cells and
HL60 cells do not; accordingly, KG1a and KG1 cells are generally considered
as the most primitive and HL60 cells as the most mature of these cell
lines. Measurement of cell adhesion to hyaluronan-coated surfaces (using
51Cr-labeled cells) and of aggregate formation in hyaluronan-containing
solutions, showed that 45% of KG1 cells and 22% to 24% of KG1a
spontaneously bind to hyaluronan, whereas HL60 cells do not either
spontaneously or after treatment with a phorbol ester. Hyaluronan binding
by KG1a and KG1 cells is mediated by CD44, because it is specifically
abolished by monoclonal antibodies (MoAbs) to this molecule. The binding
might require phosphorylation by protein kinase C and perhaps also by
protein kinase A, because it is prevented by staurosporine, which inhibits
these enzymes. 12-O- tetradecanoylphorbol-13-acetate (TPA) which activates
protein kinase C, rises to 80% the proportion of KG1 and KG1a cells that
bind hyaluronan; this activation is dependent on protein synthesis, for it
is abrogated by cyclophosphamide, a protein synthesis inhibitor. Binding of
TPA- treated cells to hyaluronan is only partly inhibited by MoAb to CD44:
this suggests that TPA may induce synthesis of a hyaluronan-binding protein
distinct from CD44. Considering the abundance of hyaluronan in human bone
marrow, these results suggest that CD44 may be involved in mediating
precursor-stroma interaction.
Volume 83,
Issue 3,
pp. 657-662,
02/01/1994
Copyright © 1994 by The American Society of Hematology
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