|
 Previous Article | Table of Contents | Next Article 
Adhesion-dependent survival of normal and leukemic human B lymphoblasts on
bone marrow stromal cells
A Manabe, KG Murti, E Coustan-Smith, M Kumagai, FG Behm, SC Raimondi and D Campana
Department of Hematology-Oncology, St Jude Children's Research Hospital,
Memphis, TN 38101.
We investigated the requirement for intimate contact between bone marrow
stroma and B lymphoblasts from normal donors and children with leukemia. By
scanning electron microscopy, both normal and leukemic cells seeded onto
stroma were surrounded by folds of stromal cells or were linked to the
stroma by fine tendrils and uropods. Separation of normal B progenitors
from stroma by use of microporous membranes led to significantly lower cell
recoveries compared with results when contact was unimpeded. For instance,
22.5% +/- 1.8% (mean +/- SEM) of CD19+, CD34+ cells (most immature subset)
were recovered after a 7-day culture directly on stroma, compared with 5.2%
+/- 0.7% after growth on membranes (P < .001 by Student's t test). In 6
of 11 cases of B-lineage acute lymphoblastic leukemia, separation of
progenitors from stroma resulted in apoptosis and a greater than 60%
reduction in cell recovery. In the remaining 5 cases, however, this effect
was much less pronounced, with reductions in cell recoveries ranging from
48.5% to less than 1% (median, 39.0%) of control values. Inhibition of very
late antigen-4, a surface molecule critical for adhesion of B lymphoblasts
to stroma, was associated with a greater loss of normal CD34+ B progenitors
compared with that for equivalent leukemic cells. These results establish
direct contact with stroma as a survival requirement of normal B
lymphoblasts and show marked heterogeneity in stromal dependency among
B-lineage leukemic cells.
Volume 83,
Issue 3,
pp. 758-766,
02/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Juarez, R. Baraz, S. Gaundar, K. Bradstock, and L. Bendall
Interaction of interleukin-7 and interleukin-3 with the CXCL12-induced proliferation of B-cell progenitor acute lymphoblastic leukemia
Haematologica,
April 1, 2007;
92(4):
450 - 459.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Mishra, B. Zhang, J. M. Cunnick, N. Heisterkamp, and J. Groffen
Resistance to imatinib of bcr/abl p190 lymphoblastic leukemia cells.
Cancer Res.,
May 15, 2006;
66(10):
5387 - 5393.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cooperman, R. Neely, D. T. Teachey, S. Grupp, and J. K. Choi
Cell Division Rates of Primary Human Precursor B Cells in Culture Reflect In Vivo Rates
Stem Cells,
November 1, 2004;
22(6):
1111 - 1120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. V. Cox, R. S. Evely, A. Oakhill, D. H. Pamphilon, N. J. Goulden, and A. Blair
Characterization of acute lymphoblastic leukemia progenitor cells
Blood,
November 1, 2004;
104(9):
2919 - 2925.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kaufmann, M. Raderer, S. Wohrer, A. Puspok, A. Bankier, C. Zielinski, A. Chott, and J. Drach
Antitumor activity of rituximab plus thalidomide in patients with relapsed/refractory mantle cell lymphoma
Blood,
October 15, 2004;
104(8):
2269 - 2271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kochling, S. A. Konig-Merediz, R. Stripecke, D. Buchwald, A. Korte, H. G. von Einsiedel, F. Sack, G. Henze, K. Seeger, B. Wittig, et al.
Protection of Mice against Philadelphia Chromosome-positive Acute Lymphoblastic Leukemia by Cell-based Vaccination Using Nonviral, Minimalistic Expression Vectors and Immunomodulatory Oligonucleotides
Clin. Cancer Res.,
August 1, 2003;
9(8):
3142 - 3149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. L. Astier, R. Xu, M. Svoboda, E. Hinds, O. Munoz, R. de Beaumont, C. D. Crean, T. Gabig, and A. S. Freedman
Temporal gene expression profile of human precursor B leukemia cells induced by adhesion receptor: identification of pathways regulating B-cell survival
Blood,
February 1, 2003;
101(3):
1118 - 1127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. M. Pedersen, S. Kitada, L. M. Leoni, J. M. Zapata, J. G. Karras, N. Tsukada, T. J. Kipps, Y. S. Choi, F. Bennett, and J. C. Reed
Protection of CLL B cells by a follicular dendritic cell line is dependent on induction of Mcl-1
Blood,
August 13, 2002;
100(5):
1795 - 1801.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Shah, L. Oseth, H. Tran, B. Hirsch, and T. W. LeBien
Clonal Variation in the B-Lineage Acute Lymphoblastic Leukemia Response to Multiple Cytokines and Bone Marrow Stromal Cells
Cancer Res.,
July 1, 2001;
61(13):
5268 - 5274.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Prosper and C. M. Verfaillie
Regulation of hematopoiesis through adhesion receptors
J. Leukoc. Biol.,
March 1, 2001;
69(3):
307 - 316.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. A. Burger, N. Tsukada, M. Burger, N. J. Zvaifler, M. Dell'Aquila, and T. J. Kipps
Blood-derived nurse-like cells protect chronic lymphocytic leukemia B cells from spontaneous apoptosis through stromal cell-derived factor-1
Blood,
October 15, 2000;
96(8):
2655 - 2663.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Todisco, T. Suzuki, K. Srivannaboon, E. Coustan-Smith, S. C. Raimondi, F. G. Behm, A. Kitanaka, and D. Campana
CD38 ligation inhibits normal and leukemic myelopoiesis
Blood,
January 15, 2000;
95(2):
535 - 542.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Cardoso, J. P. Veiga, P. Ghia, H. M. Afonso, W. N. Haining, S. E. Sallan, and L. M. Nadler
Adoptive T-Cell Therapy for B-Cell Acute Lymphoblastic Leukemia: Preclinical Studies
Blood,
November 15, 1999;
94(10):
3531 - 3540.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. G. Wickremasinghe and A. V. Hoffbrand
Biochemical and Genetic Control of Apoptosis: Relevance to Normal Hematopoiesis and Hematological Malignancies
Blood,
June 1, 1999;
93(11):
3587 - 3600.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kitanaka, T. Suzuki, C. Ito, H. Nishigaki, E. Coustan-Smith, T. Tanaka, Y. Kubota, and D. Campana
CD38-Mediated Signaling Events in Murine Pro-B Cells Expressing Human CD38 With or Without Its Cytoplasmic Domain
J. Immunol.,
February 15, 1999;
162(4):
1952 - 1958.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Miller, V. McCullar, M. Punzel, I. R. Lemischka, and K. A. Moore
Single Adult Human CD34+/Lin-/CD38- Progenitors Give Rise to Natural Killer Cells, B-Lineage Cells, Dendritic Cells, and Myeloid Cells
Blood,
January 1, 1999;
93(1):
96 - 106.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ito, M.-a. Kumagai, A. Manabe, E. Coustan-Smith, S. C. Raimondi, F. G. Behm, K. G. Murti, J. E. Rubnitz, C.-H. Pui, and D. Campana
Hyperdiploid Acute Lymphoblastic Leukemia With 51 to 65 Chromosomes: A Distinct Biological Entity With a Marked Propensity to Undergo Apoptosis
Blood,
January 1, 1999;
93(1):
315 - 320.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Shah, L. Oseth, and T. W. LeBien
Development of a Model for Evaluating the Interaction Between Human Pre-B Acute Lymphoblastic Leukemic Cells and the Bone Marrow Stromal Cell Microenvironment
Blood,
November 15, 1998;
92(10):
3817 - 3828.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Miller, V. McCullar, and C. M. Verfaillie
Ex Vivo Culture of CD34+/Lin-/DR- Cells in Stroma-Derived Soluble Factors, Interleukin-3, and Macrophage Inflammatory Protein-1alpha Maintains Not Only Myeloid But Also Lymphoid Progenitors in a Novel Switch Culture Assay
Blood,
June 15, 1998;
91(12):
4516 - 4522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Lagneaux, A. Delforge, D. Bron, C. De Bruyn, and P. Stryckmans
Chronic Lymphocytic Leukemic B Cells But Not Normal B Cells Are Rescued From Apoptosis by Contact With Normal Bone Marrow Stromal Cells
Blood,
April 1, 1998;
91(7):
2387 - 2396.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Moses, J. Nelson, and G. C. Bagby Jr
The Influence of Human Immunodeficiency Virus-1 on Hematopoiesis
Blood,
March 1, 1998;
91(5):
1479 - 1495.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. O'Prey, N. Leslie, K. Itoh, W. Ostertag, C. Bartholomew, and P. R. Harrison
Both Stroma and Stem Cell Factor Maintain Long-Term Growth of ELM Erythroleukemia Cells, but Only Stroma Prevents Erythroid Differentiation in Response to Erythropoietin and Interleukin-3
Blood,
March 1, 1998;
91(5):
1548 - 1555.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ito, R. C. Ribeiro, F. G. Behm, S. C. Raimondi, C.-H. Pui, and D. Campana
Cyclosporin A Induces Apoptosis in Childhood Acute Lymphoblastic Leukemia Cells
Blood,
February 1, 1998;
91(3):
1001 - 1007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. J. Jarvis, J. E. Maguire, and T. W. LeBien
Contact Between Human Bone Marrow Stromal Cells and B Lymphocytes Enhances Very Late Antigen-4/Vascular Cell Adhesion Molecule-1-Independent Tyrosine Phosphorylation of Focal Adhesion Kinase, Paxillin, and ERK2 in Stromal Cells
Blood,
August 15, 1997;
90(4):
1626 - 1635.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Nishigaki, C. Ito, A. Manabe, M.-a. Kumagai, E. Coustan-Smith, Y. Yanishevski, F. G. Behm, S. C. Raimondi, C.-H. Pui, and D. Campana
Prevalence and Growth Characteristics of Malignant Stem Cells in B-Lineage Acute Lymphoblastic Leukemia
Blood,
May 15, 1997;
89(10):
3735 - 3744.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Gattei, M. Degan, A. Gloghini, A. De Iuliis, S. Improta, F. M. Rossi, D. Aldinucci, V. Perin, D. Serraino, R. Babare, et al.
CD30 Ligand Is Frequently Expressed in Human Hematopoietic Malignancies of Myeloid and Lymphoid Origin
Blood,
March 15, 1997;
89(6):
2048 - 2059.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Xiao, Y. Messinger, J. Jin, D. E. Myers, J. B. Bolen, and F. M. Uckun
Signal Transduction through the beta1 Integrin Family Surface Adhesion Molecules VLA-4 and VLA-5 of Human B-cell Precursors Activates CD19 Receptor-associated Protein-tyrosine Kinases
J. Biol. Chem.,
March 29, 1996;
271(13):
7659 - 7664.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
