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The SCL/TAL-1 gene is expressed in progenitors of both the hematopoietic
and vascular systems during embryogenesis
AR Kallianpur, JE Jordan and SJ Brandt
Department of Medicine (Hematology), Vanderbilt University Medical Center,
Nashville, TN.
Activation of the SCL (or TAL-1) gene as a result of chromosomal
translocation or deletion is a frequent molecular lesion in acute T- cell
leukemia. By virtue of its membership in the basic helix-loop- helix family
of transcription factors, the SCL gene is a candidate to regulate events in
hematopoietic differentiation. We have used polyclonal antibody raised
against a bacterial expressed malE-SCL fusion protein to characterize SCL
protein expression in postimplantation embryos and in neonatal and adult
mice. SCL protein was detected at day 7.5 post coitum at both embryonic and
extraembryonic sites, approximately 24 hours before the formation of
recognizable hematopoietic elements. Expression then localized to blood
islands of the yolk sac followed by localization to fetal liver and spleen,
paralleling the hematopoietic activity of these tissues during development.
SCL protein was detected in erythroblasts in fetal and adult spleen,
myeloid cells and megakaryocytes in spleen and bone marrow, mast cells in
skin, and in rare cells in fetal and adult thymus. In addition, SCL protein
was noted in endothelial progenitors in blood islands and in endothelial
cells and angioblasts in a number of organs at times coincident with their
vascularization. SCL expression was also observed in other nonhematopoietic
cell types in the developing skeletal and nervous systems. These results
show that SCL expression is one of the earliest markers of mammalian
hematopoietic development and are compatible with a role for this
transcription factor in terminal differentiation of the erythroid and
megakaryocytic lineages. SCL expression by cells in the thymus suggests
that the gene may be active at some stage of T-cell differentiation and may
be relevant to its involvement by chromosomal rearrangements in T- lymphoid
leukemias. Finally, expression of the gene in developing brain, cartilage,
and vascular endothelium indicates SCL may have actions in neural
development, osteogenesis, and vasculogenesis, as well as in hematopoietic
differentiation.
Volume 83,
Issue 5,
pp. 1200-1208,
03/01/1994
Copyright © 1994 by The American Society of Hematology
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