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The 87-kD A gamma-globin enhancer-binding protein is a product of the
HOXB2(HOX2H) locus
PK Sengupta, DE Lavelle and J DeSimone
Department of Medicine, University of Illinois, Chicago.
Developmental regulation of globin gene expression may be controlled by
developmental stage-specific nuclear proteins that influence interactions
between the locus control region and local regulatory sequences near
individual globin genes. We previously isolated an 87-kD nuclear protein
from K562 cells that bound to DNA sequences in the beta- globin locus
control region, gamma-globin promoter, and A gamma-globin enhancer. The
presence of this protein in fetal globin-expressing cells and its absence
in adult globin-expressing cells suggested that it may be a developmental
stage-specific factor. A lambda gt11 K562 cDNA clone encoding a portion of
the HOXB2 (formerly HOX2H) homeobox gene was isolated on the basis of the
ability of its beta-galactosidase fusion protein to bind to the same DNA
sequences as the 87-kD K562 protein. Because no other relationship had been
established between the 87-kD K562 protein and the HOXB2 protein other than
their ability to bind ot the same DNA sequences, we have investigated
whether the two proteins are related antigenically. Our data show that
antisera produced against the HOXB2-beta-gal fusion protein and a synthetic
HOXB2 decapeptide react specifically with an 87-kD protein from K562
nuclear extract, showing that the 87-kD K562 nuclear protein is a product
of the HOXB2 locus, and is the first demonstration of cellular HOXB2
protein.
Volume 83,
Issue 5,
pp. 1420-1427,
03/01/1994
Copyright © 1994 by The American Society of Hematology
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