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Antigen expression and polymerase chain reaction amplification of mantle
cell lymphomas
RJ Molot, TC Meeker, CT Wittwer, SL Perkins, GH Segal, AS Masih, RC Braylan and CR Kjeldsberg
Department of Pathology, University of Utah Medical Center, Salt Lake City.
Flow immunophenotyping, DNA content analysis, and polymerase chain reaction
(PCR) amplification for t(11;14) and t(14;18) were performed on 11 cases of
typical mantle cell lymphoma (MCL), 5 cases of apparent MCL with
proliferation centers (MCL-PC), and 5 cases of small lymphocytic lymphoma
(SLL). Immunophenotyping showed IgM (P < .001), Ig light (P < .001),
and CD20 (P < .001) expression to be more intense in MCL than in SLL. In
MCL-PC, the mean intensity of IgM, Ig light chain, and CD20 expression was
intermediate to the intensities observed in MCL and SLL. Furthermore, in
contrast to SLL, all MCL and 4 of 5 MCL-PC cases exhibited stronger CD20
than CD19 expression. CD10 expression was not observed in any case and CD5
expression was present in all SLL and MCL-PC cases and in 9 of 11 MCL
cases. DNA content analysis showed an S- phase fraction of less than 3% in
all cases studied and, except for 1 MCL case, all lymphomas were DNA
diploid. The t(11;14) breakpoint junctions involving the bcl-1 major
translocation cluster were amplified by PCR in 4 of 11 (36%) MCL cases and
in none of the MCL-PC or SLL cases. The t(14;18) involving the bcl-2 major
breakpoint region was not identified by PCR in any case. We conclude that
the level of expression of surface antigens and the rapid detection of
t(11;14) by PCR are potentially useful for distinguishing MCL and SLL in
the clinical setting. Further investigations as to the biologic
relationship between MCL, MCL-PC, and SLL, and the utility of t(11;14) PCR
in these lymphomas are warranted.
Volume 83,
Issue 6,
pp. 1626-1631,
03/15/1994
Copyright © 1994 by The American Society of Hematology
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