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Inverse expression of bcl-2 protein and Fas antigen in lymphoblasts in
peripheral lymph nodes and activated peripheral blood T and B lymphocytes
T Yoshino, E Kondo, L Cao, K Takahashi, K Hayashi, S Nomura and T Akagi
Department of Pathology, Okayama University School of Medicine, Japan.
To examine the regulatory mechanism of apoptosis in lymphoid cells,
expression of both bcl-2 protein and Fas antigen was investigated in
reactive lymph nodes, in resting lymphocytes from peripheral blood (PBLs),
and in PBLs stimulated with pokeweed mitogen, interleukin-4 (IL- 4) +
anti-IgM antibody, IL-2 + anti-CD3 antibody, phytohemagglutinin + phorbol
myristate acetate using immunohistochemistry and flow cytometry. Germinal
center cells expressed a large amount of Fas antigen, which is associated
with the induction of apoptosis in lymphoid cell lines, in contrast to the
lack of bcl-2 protein as an apoptosis inhibitor. On the other hand, mantle
zone lymphocytes expressed a high level of bcl-2 protein and less Fas
antigen. This inverse expression of bcl-2 protein and Fas antigen was also
shown in activated T and B lymphocytes from peripheral blood. These
lymphoblasts fell into apoptosis dose-dependently in the presence of
anti-Fas monoclonal antibody, but resting lymphocytes that expressed both
bcl-2 protein and Fas antigen did not undergo apoptosis. These findings
suggest that bcl-2 expression prevents the apoptosis of lymphoid cells
induced by the Fas antigen-dependent mechanism and that apoptosis of
lymphocytes is exquisitely controlled, at least in part, by regulation of
the bcl-2 and Fas genes.
Volume 83,
Issue 7,
pp. 1856-1861,
04/01/1994
Copyright © 1994 by The American Society of Hematology
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