|
|
 Previous Article | Table of Contents | Next Article 
Isolation and characterization of human bone marrow microvascular
endothelial cells: hematopoietic progenitor cell adhesion
S Rafii, F Shapiro, J Rimarachin, RL Nachman, B Ferris, B Weksler, MA Moore and AS Asch
Department of Hematology-Oncology, New York Hospital-Cornell Medical
Center, New York, NY 10021.
To examine potential mechanisms by which hematopoiesis may be regulated by
endothelial cells within the bone marrow (BM) microenvironment, we have
devised a technique for the in vitro study of the interaction of human BM
microvascular endothelial cells (BMEC) with hematopoietic cells.
Microvessels isolated by collagenase digestion of spicules obtained from
filtered BM aspirate were plated on gelatin-coated plastic dishes, and
colonies of endothelial cells grown from microvessel explants were further
purified by Ulex europaeus lectin affinity separation. BMEC monolayers
isolated by this technique grew in typical cobblestone fashion, stained
positively with antibody to factor VIII/von Willebrand factor, and
incorporated acetylated LDL. Immunohistochemical studies showed that BM
microvessels and BMEC monolayers express CD34, PECAM, and thrombospondin.
Incubation of resting BMEC with BM mononuclear hematopoietic cells resulted
in the selective adhesion of relatively large numbers of CD34+ progenitor
cells and megakaryocytes. The binding of purified BM-derived CD34+
progenitor cells to BMEC was dependent on divalent cations and was
partially blocked by antibodies to CD34. IL-1 beta treatment of BMEC
monolayers resulted in an increase of CD34+ progenitor cell adhesion by
mechanisms independent of CD34 or divalent cations. BMEC exhibit specific
affinity for CD34+ progenitor cells and megakaryocytes, suggesting that the
BM microvasculature may play a role in regulating the trafficking,
proliferation, and differentiation of lineage specific hematopoietic
elements, and possibly of pluripotent stem cells within the CD34+
population.
Volume 84,
Issue 1,
pp. 10-19,
07/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. Ria, C. Piccoli, T. Cirulli, F. Falzetti, G. Mangialardi, D. Guidolin, A. Tabilio, N. Di Renzo, A. Guarini, D. Ribatti, et al.
Endothelial Differentiation of Hematopoietic Stem and Progenitor Cells from Patients with Multiple Myeloma
Clin. Cancer Res.,
March 15, 2008;
14(6):
1678 - 1685.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Chute, G. G. Muramoto, A. B. Salter, S. K. Meadows, D. W. Rickman, B. Chen, H. A. Himburg, and N. J. Chao
Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated mice
Blood,
March 15, 2007;
109(6):
2365 - 2372.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-L. Lin, D. Chang, and S.-Y. Ying
Hyaluronan stimulates transformation of androgen-independent prostate cancer
Carcinogenesis,
February 1, 2007;
28(2):
310 - 320.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Boos, G. Y.H. Lip, and A. D. Blann
Circulating Endothelial Cells in Cardiovascular Disease
J. Am. Coll. Cardiol.,
October 17, 2006;
48(8):
1538 - 1547.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Chute, G. G. Muramoto, H. K. Dressman, G. Wolfe, N. J. Chao, and S. Lin
Molecular Profile and Partial Functional Analysis of Novel Endothelial Cell-Derived Growth Factors that Regulate Hematopoiesis
Stem Cells,
May 1, 2006;
24(5):
1315 - 1327.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. Howson, A. C. Aplin, M. Gelati, G. Alessandri, E. A. Parati, and R. F. Nicosia
The postnatal rat aorta contains pericyte progenitor cells that form spheroidal colonies in suspension culture
Am J Physiol Cell Physiol,
December 1, 2005;
289(6):
C1396 - C1407.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-G. Kopp, S. T. Avecilla, A. T. Hooper, and S. Rafii
The Bone Marrow Vascular Niche: Home of HSC Differentiation and Mobilization
Physiology,
October 1, 2005;
20(5):
349 - 356.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-G. Kopp, S. T. Avecilla, A. T. Hooper, S. V. Shmelkov, C. A. Ramos, F. Zhang, and S. Rafii
Tie2 activation contributes to hemangiogenic regeneration after myelosuppression
Blood,
July 15, 2005;
106(2):
505 - 513.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-C. Chen, C.-C. Huang, C.-L. Chien, C.-J. Jeng, H.-T. Su, E. Chiang, M.-R. Liu, C. H. H. Wu, C.-N. Chang, and R.-H. Lin
Cross-linking of P-selectin glycoprotein ligand-1 induces death of activated T cells
Blood,
November 15, 2004;
104(10):
3233 - 3242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Hermann, R. Gastl, S. Liebau, M. O. Popa, J. Fiedler, B. O. Boehm, M. Maisel, H. Lerche, J. Schwarz, R. Brenner, et al.
Efficient generation of neural stem cell-like cells from adult human bone marrow stromal cells
J. Cell Sci.,
September 1, 2004;
117(19):
4411 - 4422.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Bompais, J. Chagraoui, X. Canron, M. Crisan, X. H. Liu, A. Anjo, C. Tolla-Le Port, M. Leboeuf, P. Charbord, A. Bikfalvi, et al.
Human endothelial cells derived from circulating progenitors display specific functional properties compared with mature vessel wall endothelial cells
Blood,
April 1, 2004;
103(7):
2577 - 2584.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Moldenhauer, M. Nociari, G. Lam, A. Salama, S. Rafii, and M. A.S. Moore
Tumor Necrosis Factor Alpha-Stimulated Endothelium: An Inducer of Dendritic Cell Development from Hematopoietic Progenitors and Myeloid Leukemic Cells
Stem Cells,
March 1, 2004;
22(2):
144 - 157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Chute, G. Muramoto, J. Fung, and C. Oxford
Quantitative Analysis Demonstrates Expansion of SCID-Repopulating Cells and Increased Engraftment Capacity in Human Cord Blood Following Ex Vivo Culture with Human Brain Endothelial Cells
Stem Cells,
March 1, 2004;
22(2):
202 - 215.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Schuch, J. V. Heymach, M. Nomi, M. Machluf, J. Force, A. Atala, J. P. Eder Jr., J. Folkman, and S. Soker
Endostatin Inhibits the Vascular Endothelial Growth Factor-Induced Mobilization of Endothelial Progenitor Cells
Cancer Res.,
December 1, 2003;
63(23):
8345 - 8350.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Netelenbos, J. van den Born, F. L. Kessler, S. Zweegman, P. C. Huijgens, and A. M. Drager
In vitro model for hematopoietic progenitor cell homing reveals endothelial heparan sulfate proteoglycans as direct adhesive ligands
J. Leukoc. Biol.,
December 1, 2003;
74(6):
1035 - 1044.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Vacca, R. Ria, F. Semeraro, F. Merchionne, M. Coluccia, A. Boccarelli, C. Scavelli, B. Nico, A. Gernone, F. Battelli, et al.
Endothelial cells in the bone marrow of patients with multiple myeloma
Blood,
November 1, 2003;
102(9):
3340 - 3348.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Zhang, N. R. Hackett, G. Lam, J. Cheng, R. Pergolizzi, L. Luo, S. V. Shmelkov, J. Edelberg, R. G. Crystal, and S. Rafii
Green fluorescent protein selectively induces HSP70-mediated up-regulation of COX-2 expression in endothelial cells
Blood,
September 15, 2003;
102(6):
2115 - 2121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Dazzi, A. Cariello, P. Giovanis, M. Monti, B. Vertogen, M. Leoni, A. Tienghi, D. Turci, G. Rosti, O. Nanni, et al.
Prophylaxis with GM-CSF mouthwashes does not reduce frequency and duration of severe oral mucositis in patients with solid tumors undergoing high-dose chemotherapy with autologous peripheral blood stem cell transplantation rescue: a double blind, randomized, placebo-controlled study
Ann. Onc.,
April 1, 2003;
14(4):
559 - 563.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S L Preston, M R Alison, S J Forbes, N C Direkze, R Poulsom, and N A Wright
The new stem cell biology: something for everyone
Mol. Pathol.,
April 1, 2003;
56(2):
86 - 96.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-L. Hillebrands, F. A. Klatter, and J. Rozing
Origin of Vascular Smooth Muscle Cells and the Role of Circulating Stem Cells in Transplant Arteriosclerosis
Arterioscler. Thromb. Vasc. Biol.,
March 1, 2003;
23(3):
380 - 387.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Chute, A. A. Saini, D. J. Chute, M. R. Wells, W. B. Clark, D. M. Harlan, J. Park, M. K. Stull, C. Civin, and T. A. Davis
Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrow
Blood,
December 15, 2002;
100(13):
4433 - 4439.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Simper, P. G. Stalboerger, C. J. Panetta, S. Wang, and N. M. Caplice
Smooth Muscle Progenitor Cells in Human Blood
Circulation,
September 3, 2002;
106(10):
1199 - 1204.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. E. Bolch, P. W. Patton, D. A. Rajon, A. P. Shah, D. W. Jokisch, and B. A. Inglis
Considerations of Marrow Cellularity in 3-Dimensional Dosimetric Models of the Trabecular Skeleton
J. Nucl. Med.,
January 1, 2002;
43(1):
97 - 108.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Dimitroff, J. Y. Lee, S. Rafii, R. C. Fuhlbrigge, and R. Sackstein
CD44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells
J. Cell Biol.,
June 11, 2001;
153(6):
1277 - 1286.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Hattori, B. Heissig, K. Tashiro, T. Honjo, M. Tateno, J.-H. Shieh, N. R. Hackett, M. S. Quitoriano, R. G. Crystal, S. Rafii, et al.
Plasma elevation of stromal cell-derived factor-1 induces mobilization of mature and immature hematopoietic progenitor and stem cells
Blood,
June 1, 2001;
97(11):
3354 - 3360.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Voermans, P.M.L. Rood, P.L. Hordijk, W.R. Gerritsen, and C.E. van der Schoot
Adhesion Molecules Involved in Transendothelial Migration of Human Hematopoietic Progenitor Cells
Stem Cells,
November 1, 2000;
18(6):
435 - 443.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
B. Dankbar, T. Padro, R. Leo, B. Feldmann, M. Kropff, R. M. Mesters, H. Serve, W. E. Berdel, and J. Kienast
Vascular endothelial growth factor and interleukin-6 in paracrine tumor-stromal cell interactions in multiple myeloma
Blood,
April 15, 2000;
95(8):
2630 - 2636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Schatz, C. Soderland, K. D. Hendricks-Muñoz, R. P. Gerrets, and C. J. Lockwood
Human Endometrial Endothelial Cells: Isolation, Characterization, and Inflammatory-Mediated Expression of Tissue Factor and Type 1 Plasminogen Activator Inhibitor
Biol Reprod,
March 1, 2000;
62(3):
691 - 697.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Peichev, A. J. Naiyer, D. Pereira, Z. Zhu, W. J. Lane, M. Williams, M. C. Oz, D. J. Hicklin, L. Witte, M. A. S. Moore, et al.
Expression of VEGFR-2 and AC133 by circulating human CD34+ cells identifies a population of functional endothelial precursors
Blood,
February 1, 2000;
95(3):
952 - 958.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gutiérrez-Rodríguez, E. Reyes-Maldonado, and H. Mayani
Characterization of the Adherent Cells Developed in Dexter-Type Long-Term Cultures from Human Umbilical Cord Blood
Stem Cells,
January 1, 2000;
18(1):
46 - 52.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. J. Naiyer, D.-Y. Jo, J. Ahn, R. Mohle, M. Peichev, G. Lam, R. L. Silverstein, M. A.S. Moore, and S. Rafii
Stromal Derived Factor-1-Induced Chemokinesis of Cord Blood CD34+ Cells (Long-Term Culture-Initiating Cells) Through Endothelial Cells Is Mediated by E-Selectin
Blood,
December 15, 1999;
94(12):
4011 - 4019.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A Fontes and A. W Thomson
Stem cell technology
BMJ,
November 13, 1999;
319(7220):
1308 - 1308.
[Full Text]
|
|
|
|
|
|
|
K. Imai, M. Kobayashi, J. Wang, Y. Ohiro, J.-i. Hamada, Y. Cho, M. Imamura, M. Musashi, T. Kondo, M. Hosokawa, et al.
Selective Transendothelial Migration of Hematopoietic Progenitor Cells: A Role in Homing of Progenitor Cells
Blood,
January 1, 1999;
93(1):
149 - 156.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hamada, R. Mohle, J. Hesselgesser, J. Hoxie, R. L. Nachman, M. A.S. Moore, and S. Rafii
Transendothelial Migration of Megakaryocytes in Response to Stromal Cell-derived Factor 1 (SDF-1) Enhances Platelet Formation
J. Exp. Med.,
August 3, 1998;
188(3):
539 - 548.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Yong, M. Watts, N. Shaun Thomas, A. Sullivan, S. Ings, and D. C. Linch
Transmigration of CD34+ Cells Across Specialized and Nonspecialized Endothelium Requires Prior Activation by Growth Factors and Is Mediated by PECAM-1 (CD31)
Blood,
February 15, 1998;
91(4):
1196 - 1205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Cardier and J. Dempsey
Thrombopoietin and Its Receptor, c-mpl, Are Constitutively Expressed by Mouse Liver Endothelial Cells: Evidence of Thrombopoietin as a Growth Factor for Liver Endothelial Cells
Blood,
February 1, 1998;
91(3):
923 - 929.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-Y. Liu, R. K. Ganju, J.-F. Wang, K. Schweitzer, B. Weksler, S. Avraham, and J. E. Groopman
Characterization of Signal Transduction Pathways in Human Bone Marrow Endothelial Cells
Blood,
September 15, 1997;
90(6):
2253 - 2259.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Garlanda and E. Dejana
Heterogeneity of Endothelial Cells : Specific Markers
Arterioscler. Thromb. Vasc. Biol.,
July 1, 1997;
17(7):
1193 - 1202.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. Mohle, M. A.S. Moore, R. L. Nachman, and S. Rafii
Transendothelial Migration of CD34+ and Mature Hematopoietic Cells: An In Vitro Study Using a Human Bone Marrow Endothelial Cell Line
Blood,
January 1, 1997;
89(1):
72 - 80.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M Schattner, P Lefebvre, S. Mingolelli, C. Goolsby, A Rademaker, J. White, D Foster, D Green, and I Cohen
Thrombopoietin-stimulated ex vivo expansion of human bone marrow megakaryocytes
Stem Cells,
March 1, 1996;
14(2):
207 - 214.
[Abstract]
|
|
|
|
|
|
|
S. Rafii, M. C. Oz, J. A. Seldomridge, B. Ferris, A. S. Asch, R. L. Nachman, F. Shapiro, E. A. Rose, and H. R. Levin
Characterization of Hematopoietic Cells Arising on the Textured Surface of Left Ventricular Assist Devices
Ann. Thorac. Surg.,
December 1, 1995;
60(6):
1627 - 1632.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Dickson, J. Martin, F. Cousins, A. Kulkarni, S Karlsson, and R. Akhurst
Defective haematopoiesis and vasculogenesis in transforming growth factor-beta 1 knock out mice
Development,
January 6, 1995;
121(6):
1845 - 1854.
[Abstract]
[PDF]
|
|
|
|
