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Effects of butyrate on the erythropoietin receptor of cell line IW201
O Hermine, YJ Dong and E Goldwasser
Department of Biochemistry and Molecular Biology, University of Chicago, IL
60637.
The murine erythroleukemic cell line, IW201, normally expresses only
low-affinity erythropoietin receptors. Exposure of these cells for 48 hours
to sodium butyrate results in a change in receptor kd from about 600 pmol/L
to 100 to 200 pmol/L. This change in affinity is accompanied by
downregulation of both receptor number and receptor mRNA. Cells exposed to
sodium butyrate for 2 hours show a similar change in kd but no change in
receptor number. The butyrate effect on kd at 2 hours is abrogated by
either cycloheximide or actinomycin D. These data indicate that an
accessory protein induced by sodium butyrate is responsible for
high-affinity binding of erythropoietin.
Volume 84,
Issue 3,
pp. 811-814,
08/01/1994
Copyright © 1994 by The American Society of Hematology
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