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The sialomucin CD34 is expressed on hematopoietic cells and blood vessels
during murine development
PE Young, S Baumhueter and LA Lasky
Department of Immunology, Genentech, Inc, South San Francisco, CA 94080.
The processes of angiogenesis and hematopoiesis require a high degree of
coordination during embryogenesis. Whereas much is understood about the
development of the vascular system in avian embryos, little information has
been attained in mammals, predominantly because there are no specific
markers for either blood vessels or hematopoietic cells in any developing
mammalian system. We have recently shown that murine CD34 (mCD34) is
expressed on the vascular endothelium in all organs and tissues of the
adult mouse as well as on a small percentage of presumably hematopoietic
stem cells in the bone marrow and fetal liver. Here we show that mCD34 is
also expressed on the endothelium of blood vessels and on a subset of
hematopoietic-like cells throughout murine development. mCD34 is first
observed on the yolk sac endothelium of day 7.5 embryos and on a subset of
hematopoietic cells within these yolk sacs. mCD34 expression is maintained
on vessels and hematopoietic cells in all organs and tissues throughout
embryogenesis. In addition, mCD34 is localized on growth conelike
filopodial processes that appear at the budding edge of newly sprouted
capillaries. Double staining of capillaries for mCD34 and laminin shows
that these growth conelike processes seem to be free of laminin, whereas
the formed capillaries seem to be coated with this extracellular matrix
protein. Analysis of vessels in developing brain shows that these
filopodial processes seem to be directed toward the ventricular epithelium,
a previously described site of vascular endothelial growth factor
synthesis. Finally, we show that the vascular structures of developing
murine embryoid bodies also express mCD34. These data suggest that mCD34 is
a useful marker for the analysis of the development of the blood vascular
system in murine embryos.
Volume 85,
Issue 1,
pp. 96-105,
01/01/1995
Copyright © 1995 by The American Society of Hematology
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