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Purification and characterization of a C5a-inactivating enzyme from human
peritoneal fluid
SK Ayesh, Y Azar, II Barghouti, JM Ruedi, BM Babior and Y Matzner
Hematology Unit, Hadassah University Hospital, Mount Scopus, Jerusalem,
Israel.
Earlier work has suggested that familial Mediterranean fever, an inherited
disorder characterized by sporadic episodes of inflammation involving the
pleural and peritoneal cavities and the joints, is caused by the lack of a
C5a inactivator normally found in serosal fluid. We have purified this
inactivator from ascites fluid and obtained a protein of molecular weight
53 to 56 kD with a specific activity 10,000- fold greater than the crude
material. On Western blot, an inhibitory antibody recognized a single
antigenic species at the same molecular weight. The enzyme had no activity
against denatured bovine serum albumin. With recombinant C5a as substrate,
the Km and Vm were 3.4 mumol/L and 52 nmol C5a/min/mg protein,
respectively.
Volume 85,
Issue 12,
pp. 3503-3509,
06/15/1995
Copyright © 1995 by The American Society of Hematology
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