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Lymphokine requirement for the generation of natural killer cells from
CD34+ hematopoietic progenitor cells
A Shibuya, K Nagayoshi, K Nakamura and H Nakauchi
Division of Hematology, University of Tsukuba, Japan.
We have established a cell culture system without stromal cells that allows
the CD34+ hematopoietic progenitor cells (HPC) to differentiate into
natural killer (NK) cells. CD34+Lin (CD3, CD16, CD56)- cells were purified
using fluorescence-activated cell sorting from normal adult bone marrow
(BM) and cultured for 28 days in medium supplemented with interleukin-2
(IL-2) and stem cell factor (SCF). NK (CD3-CD16-CD56+) cells were generated
in a dose-dependent manner in response to SCF. NK cells originated from
CD34+CD33+Lin- cells, but they were barely detectable in cultures of
CD34+CD33-Lin- cells. However, on addition of IL-3, an induced
differentiation of NK cells from CD34+CD33-Lin- cells was observed,
although at a lower frequency. Supplementing of the cell cultures with SCF
alone or both SCF and IL-3 for the first 7 days followed by IL-2 for the
next 21 days is essential for production of NK cells from CD34+CD33+Lin-
cells and from CD34+CD33-Lin- cells, respectively. These data provide
direct evidence that NK cells arise from CD34+HPC and show the minimum
lymphokine requirement for their differentiation.
Volume 85,
Issue 12,
pp. 3538-3546,
06/15/1995
Copyright © 1995 by The American Society of Hematology
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