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Interleukin-5 signaling in human eosinophils involves JAK2 tyrosine kinase
and Stat1 alpha
T van der Bruggen, E Caldenhoven, D Kanters, P Coffer, JA Raaijmakers, JW Lammers and L Koenderman
Department of Pulmonary Diseases, University Hospital, Utrecht, The
Netherlands.
Signaling by a wide variety of cytokines, including interferons,
interleukins, and growth factors, involves activation of JAK kinases and
Stat (Signal transducers and activators of transcription) proteins. At
present, not much is known about the molecular mechanisms by which
interleukin-5 (IL-5) exerts its diverse biologic effects. Human eosinophils
are one of the most important target cells for IL-5 and were used here to
study IL-5 signaling in a primary human cell. IL-5 induced rapid and
transient tyrosine phosphorylation of JAK2. Moreover, IL-5 induced at least
two DNA-binding complexes, using nuclear extracts from normal human
eosinophils and the IL-6/interferon-gamma response element of the ICAM-1
promoter (ICAM-1 pIRE) in an electromobility shift assay. From supershift
experiments it was concluded that one DNA- binding complex contained Stat1
alpha, probably as a homodimer. Both DNA-binding complexes were inhibited
by a phosphotyrosine antibody (4G10), suggesting that tyrosine
phosphorylation is required for complex formation. IL-3 and
granulocyte-macrophage colony-stimulating factor induced, similar to IL-5,
two DNA-binding complexes in human eosinophils, including Stat1 alpha.
These data show for the first time that molecular mechanisms of IL-5
signaling in human eosinophils involve members of the JAK kinase family as
well as members of the Stat family.
Volume 85,
Issue 6,
pp. 1442-1448,
03/15/1995
Copyright © 1995 by The American Society of Hematology
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|
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|
|
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|
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|
|
|
|
|
|
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|
|
|
|
|
|
|
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[Full Text]
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|
|
|
|
|
|
|
P. Wang, P. Wu, B. Cheewatrakoolpong, J. G. Myers, R. W. Egan, and M. M. Billah
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160(9):
4427 - 4432.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Okada, J. B. Hagan, M. Kato, J. L. Bankers-Fulbright, L. W. Hunt, G. J. Gleich, and H. Kita
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J. Immunol.,
April 15, 1998;
160(8):
4010 - 4017.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Ogata, T. Kouro, A. Yamada, M. Koike, N. Hanai, T. Ishikawa, and K. Takatsu
JAK2 and JAK1 Constitutively Associate With an Interleukin-5 (IL-5) Receptor alpha and beta c Subunit, Respectively, and Are Activated Upon IL-5 Stimulation
Blood,
April 1, 1998;
91(7):
2264 - 2271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Bates, W. W. Busse, and P. J. Bertics
Interleukin 5 Signals through Shc and Grb2 in Human Eosinophils
Am. J. Respir. Cell Mol. Biol.,
January 1, 1998;
18(1):
75 - 83.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. I. Wahl, A.-C. Fluckiger, R. M. Kato, H. Park, O. N. Witte, and D. J. Rawlings
Phosphorylation of two regulatory tyrosine residues in the activation of Bruton's tyrosine kinase via alternative receptors
PNAS,
October 14, 1997;
94(21):
11526 - 11533.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kirito, M. Uchida, M. Yamada, Y. Miura, and N. Komatsu
A Distinct Function of STAT Proteins in Erythropoietin Signal Transduction
J. Biol. Chem.,
June 27, 1997;
272(26):
16507 - 16513.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. P. de Groot, T. B. van Dijk, E. Caldenhoven, P. J. Coffer, J. A.M. Raaijmakers, J.-W. J. Lammers, and L. Koenderman
Activation of 12-O-Tetradecanoylphorbol-13-acetate Response Element- and Dyad Symmetry Element-dependent Transcription by Interleukin-5 Is Mediated by Jun N-terminal Kinase/Stress-activated Protein Kinase Kinases
J. Biol. Chem.,
January 24, 1997;
272(4):
2319 - 2325.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Caldenhoven
STAT3beta , a Splice Variant of Transcription Factor STAT3, Is a Dominant Negative Regulator of Transcription
J. Biol. Chem.,
May 24, 1996;
271(22):
13221 - 13227.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. F. Brizzi, M. G. Aronica, A. Rosso, G. P. Bagnara, Y. Yarden, and L. Pegoraro
Granulocyte-Macrophage Colony-stimulating Factor Stimulates JAK2 Signaling Pathway and Rapidly Activates p93[IMAGE], STAT1 p91, and STAT3 p92 in Polymorphonuclear Leukocytes
J. Biol. Chem.,
February 16, 1996;
271(7):
3562 - 3567.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Caldenhoven, T. van Dijk, J. A. M. Raaijmakers, J.-W. J. Lammers, L. Koenderman, and R. P. de Groot
Activation of the STAT3/Acute Phase Response Factor Transcription Factor by Interleukin-5
J. Biol. Chem.,
October 27, 1995;
270(43):
25778 - 25784.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Du, Y. M. Alsayed, F. Xin, S. J. Ackerman, and L. C. Platanias
Engagement of the CrkL Adapter in Interleukin-5 Signaling in Eosinophils
J. Biol. Chem.,
October 13, 2000;
275(42):
33167 - 33175.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Roy, M. Audette, and M. J. Tremblay
Intercellular Adhesion Molecule-1 (ICAM-1) Gene Expression in Human T Cells Is Regulated by Phosphotyrosyl Phosphatase Activity. INVOLVEMENT OF NF-kappa B, Ets, AND PALINDROMIC INTERFERON-gamma -RESPONSIVE ELEMENT-BINDING SITES
J. Biol. Chem.,
April 27, 2001;
276(18):
14553 - 14561.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
