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Murine hematopoietic stem and progenitor cells: I. Enrichment and biologic
characterization
CL Li and GR Johnson
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne
Hospital, Victoria, Australia.
Murine bone marrow cells were fractionated by fluorescence-activated cell
sorting into Rh123lo Lin- c-kit+ Ly6A+, Rh123hi Lin-c-kit+ Ly6A+, and Lin-
c-kit+ Ly6A- populations within which most, if not all, of the
hematopoietic activities of the marrow resided. The Rh123lo Lin- c-
kit+Ly6A+ cells, which consist exclusively of small- or medium-sized
lymphocyte-like cells, are highly enriched for long-term hematopoietic in
vivo repopulating cells. The enrichment factor for these cells from the
marrow was estimated as 2,000-fold. The Rh123hi Lin- c-kit+ Ly6A+ cells,
although also highly enriched for day-12 spleen colony-forming units, were
relatively depleted of long-term in vivo repopulation capacity. Most, if
not all Lin- c-kit+ Ly6A- cells were Rb123hi. In contrast to both Rh123lo
and Rh123hi Lin- c-kit+ Ly6A+ stem cell populations, the Lin- c-kit+ Ly6A-
cells can be stimulated to proliferate in vitro in the presence of single
cytokines, which is a characteristic of committed progenitor cells. No
marked synergistic interactions between individual cytokines were observed
with this cell population. Both Rh123hi Lin- c-kit+ Ly6A+ mature stem cell
and Lin- c- kit+ Ly6A- progenitor cell populations displayed in vivo
repopulation kinetics resembling those of the putative short-term
hematopoietic repopulating cells.
Volume 85,
Issue 6,
pp. 1472-1479,
03/15/1995
Copyright © 1995 by The American Society of Hematology
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