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Cord blood T lymphocytes lack constitutive perforin expression in contrast
to adult peripheral blood T lymphocytes
C Berthou, S Legros-Maida, A Soulie, A Wargnier, J Guillet, C Rabian, E Gluckman and M Sasportes
INSERM U 93, Laboratory of Immunology and Histocompatibility, St Louis
Hospital, Paris, France.
Perforin is the cytolytic pore-forming protein, which alone can be
responsible for the lethal hit in one of the killing mechanisms used by
natural killer (NK) cells or cytotoxic T lymphocytes. In this study,
perforin expression was investigated in cord blood (CB) lymphocytes to
determine their killing potential in vivo. The majority of CB CD3- NK cells
had the protein. Compared with adult perforin-positive NK cells, a
significantly lower percentage of cells expressing CD56 and CD57, the
related neural cell adhesion molecules, was found (P = .0001). Perforin was
also present in a unique immature CB NK-cell subset, characterized by
cytoplasmic CD3 antigen (Ag) expression. In CB, very few CD8
perforin-positive T lymphocytes could be detected, but they were in
significant numbers in adult peripheral blood (P = .02). A substantial
proportion of these cells (70% +/- 23%) lacked the CD28 T-cell coactivation
Ag, and they were able to exert NK-like, major histocompatibility complex
nonrestricted cytolytic activity. CD4+ and gamma delta-T cells expressing
perforin were absent from CB, but low numbers of such cells were detected
in adult peripheral blood (P = .0001). Therefore, the spontaneous cytolytic
activity of CB lymphocytes appeared to be dependent on well-represented
perforin-positive NK cells, which were shown to efficiently lyse
NK-sensitive target cells.
Volume 85,
Issue 6,
pp. 1540-1546,
03/15/1995
Copyright © 1995 by The American Society of Hematology
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