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Recognition of human T-leukemia virus (HTLV-1) envelope by human CD4+ T-
cell lines from HTLV-1 seronegative individuals: specificity and clonal
heterogeneity
F Manca, G Li Pira, D Fenoglio, MT Valle, A Kunkl, A Ferraris, F Lancia, D Saverino, L Mortara and R Balderas
Department of Immunology, San Martino Hospital, University of Genoa, Italy.
Because T-helper cells are critical for immune responses in retroviral
infections, CD4+ T-cell lines specific for the human T-leukemia virus type
1 (HTLV-1) envelope have been generated from peripheral T lymphocytes of
nonimmune donors to study their naive repertoire. Recombinant fragments
(RE1, amino acids [aa] 26-200; RE3, aa 165-307; RE5, aa 308-401; and RE6,
aa 165-401) of HTLV-1 envelope, whole envelope glycoprotein, and synthetic
peptides were used to induce T- cell lines. CD4+ T-cell lines specific for
one or more fragments were obtained from seven of eight individuals tested.
T-cell lines generated against envelope glycoprotein from five of five
donors did not cross- react with the RE fragments and vice versa. The lines
specific for RE and env were mapped with overlapping peptides. The lines
with single peptide (narrow) specificity contained a variety of clones that
used different T-cell receptor V beta genes. These data (1) suggest that
most of the normal individuals carry T-helper precursors specific for
epitopes on HTLV-1 envelope; (2) indicate that heterogeneity of HTLV-1
envelope-specific T cells can be detected in the naive repertoire; and (3)
define optimal antigenic preparations to be used to assess cellular
immunity in HTLV-1-infected individuals.
Volume 85,
Issue 6,
pp. 1547-1554,
03/15/1995
Copyright © 1995 by The American Society of Hematology
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