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Demonstration that thiazole-orange-positive platelets in the dog are less
than 24 hours old
GL Dale, P Friese, LA Hynes and SA Burstein
W.K. Warren Medical Research Institute, Oklahoma City, OK, USA.
Approximately 6% of dog platelets are positive for staining with thiazole
orange, a dye frequently used to stain ribonucleic acid. In this report,
thiazole-orange positivity is shown to mark platelets that are less than 24
hours old. Dog platelets were derivatized in vivo with N-hydroxysuccinimido
biotin such that greater than 95% of all platelets were biotinylated. Newly
synthesized, nonbiotinylated platelets were then monitored by flow
cytometry for their ability to bind thiazole orange. After biotinylation,
the percentage of biotin-negative, thiazole-orange-positive platelets
increased gradually from 0.72% at 30 minutes to 5.44% at 24 hours. These
data indicate that thiazole-orange staining does label newly synthesized
platelets.
Volume 85,
Issue 7,
pp. 1822-1825,
04/01/1995
Copyright © 1995 by The American Society of Hematology
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